定量检测膀胱尿路上皮癌患者手术前后外周血中survivin mRNA的临床意义

Clinical significance of survivin mRNA detection in the peripheral whole blood of patients with bladder urothelial carcinoma before and after surgery

目的:研究膀胱移行细胞癌患者手术前后全血中survivin mRNA表达变化及其临床意义。方法:采用qRT-PCR检测47名非肌层浸润膀胱癌患者与25名正常人群手术前后外周血中survivin mRNA表达量并构建受试者工作特征(receiver operating characteristic,ROC)曲线。结果:术前膀胱癌患者survivin mRNA表达水平是正常人群的4.06倍(P<0.0 5),经ROC曲线分析,区别膀胱癌患者与正常人群的sur vivin表达临界值为1.4×10^(-2),患者组术后各时间点survivin表达水平明显低于术前水平(P<0.05),survivin mRNA水平高于临界值的术后患者较低于临界值的患者复发率高(P<0.05)。结论:qRT-PCR可简单敏感地检测全血中survivin mRNA水平,survivin可作为用于膀胱癌诊断及复发判断的标志物。

Objective： To investigate the mRNA expression of survivin in whole blood of patients with bladder urothelial carcinoma. Methods： Levels of survivin mRNA expression in the whole blood of 47 patients with non-muscleinvasive bladder cancer before and after operations and 25 normal controls were detected by qRT-PCR and the receiver operating characteristic（ROC） curve for each factor was constructed. Results： The levels of survivin mRNA in bladder cancer patients were 4.06 folds compared to normal controls（P〈0.05）. The cutoff value for differentiating bladder cancer patients and normal people was 1.4×10-2 for survivin. Postoperative survivin expression in patients was significantly lower than preoperative expression at each time point（P〈0.05）. Post-operative recurrence rate of post-operative patients with mRNA level above cutoff value were higher than lower ones（P〈0.05）. Conclusion： qRT-PCR is a convenient and sensitive method to detect survivin mRNA in the whole blood using, and survivin could be useful tumor biomarkers for early detection and recurrence of bladder cancer.