当归不同药用部位对溶血性血虚大鼠的补血作用

Enriching blood function of different medicinal part in Angelica sinensis on the hemolytic blood deficiency in rats

目的研究甘肃产当归不同药用部位对溶血性血虚大鼠作用的异同。方法用乙酰苯肼复制溶血性血虚大鼠模型。按照体重将Wistar大鼠随机分为6组,每组10只:空白组、模型组、阳性对照组(驴胶补血颗粒:3.60g·kg^(-1))、全归组、归身组、归尾组(均10.8 g·kg^(-1))。空白组与模型组给予0.9%Na Cl 20 m L·kg^(-1),各给药组给予相应的水煎液,均灌胃给药,每天1次,连续给药12 d。用血细胞分析仪测定大鼠全血中红细胞(RBC)、红细胞压积(HCT)、血红蛋白浓度(HGB)、血小板(PLT)、红细胞平均容积(MCV)、红细胞平均血红蛋白量(MCH)、红细胞平均血红蛋白浓度(MCHC),计算肝指数(LI)、胸腺指数(TI)和脾指数(SI)、网织红细胞计数(RC)、骨髓有核细胞计数;收集股骨,用流式细胞仪检测骨髓细胞周期。结果与模型组比较,全归组和归身组的RBC、HGB、HCT、PLT、MCHC的含量分别为(6.05±0.22),(6.12±0.12)10^(12)/L;(155.80±4.89)%,(154.40±5.19)%;(53.79±1.88),(53.27±1.57)g·L^(-1);(68.21±5.63),(67.53±5.11)10^(10)/L;(289.70±3.94),(289.91±8.41)g·L^(-1),差异均有统计学意义(P<0.05,P<0.01);与模型组比较,全归组、归身组大鼠的MCH、MCV的含量分别为(25.75±0.44),(25.23±1.09)pg;(88.88±1.67),(87.01±2.72)fl,差异均有统计学意义(均P<0.05)。与模型组比较,归尾组的RBC、HCT、HGB的含量分别为(5.69±0.40)10^(12)/L,(123.10±3.70)%,(51.41±2.03)g·L^(-1),差异均有统计学意义(均P<0.05)。结论全归和归身对溶血性血虚大鼠具有补血作用,两者补血作用相当;且全归和归身补血强于归尾。

Objective To study the difference effects of enriching blood function of different medicinal parts in Angelica on the hemolytic blood deficiency in rats. Methods Wistar rats were randomly divided into 6 groups ： blank group, model group, positive control group, Angelica group, Angelica body group, Angelica tail group. The hemolytic blood deficiency rats was established by acetyl phenylhydraziue hydrochloride. Rats in normal and model groups were orally administered with saline, while rats in positive control group were orally treated with Lvjiaobuxue granule at the dose of 3.60 g·kg-1. Water decoction of Angelica, An- gelica body and Angelica tail groups were orally at the dose of 10. 8 g· kg-1. All the drugs were administered to rats for consecutive 12 d. The contents of red blood cell （ RBC ）, hematocrit （ HCT）, hemoglobin （ HGB ） , platelet （ PLT ） , mean corpuscular volume （ MCV ） , meancorpuscular hemoglobin（MCH） and mean corpuscular hemoglobin concentration（MCHC） were detected by hematology analyzer. While liver index（ LI）, thymus index （ TI）, spleen index （SI） , reticulocyte （ RC ） , and marrow karyocyte were calculated. After collecting thighbone, marrow cell cycle was detected by flow cytometry method flow cytometry. Results With the treatment of Angelica and Angelica body water decoction, the whole blood contents of RBC, HGB, HCT, PLT, MCHC were（6.05 ± 0.22）, （6.12 ±0.12） 10 2/L; （155.80±4.89）%, （154.40 ± 5.19 ）%; （53.79±1.88）,（53.27 ± 1.57） g·L-1; （68.21± 5.63）, （67.53± 5.11） 1010/L; （289.70 ± 3.94）, （289.91 ±8.41 ） g·L - 1, respectively in each group, which were obviously higher than that of model group （ P 〈 0. 05, P 〈 0.01 ）. The contents of MCH, MCV in Angelica and Angelica body groups were （25.75± 0.44）, （25.23 ±1.09） pg; （88.88 ± 1.67）, （87.01 ±2.72） fl, which were lower than that of model group（all P 〈0.05）. After used by Angelica tail, the contents of RBC, HGB, HCT were （5.69±0. 40） 1012/L, （ 123.10 ± 3.70） %, （51.41 ± 2. 03 ） g· L- 1, which were higher than that of model group （ all P 〈 0. 05 ）. Conclusion Angelica and Angelica body, in which has same enrichment blood effect has effect of enriching blood on the hemolytic blood deficiency rats, and Angelic tail has weaker effect of enriching blood.