四逆散对创伤后应激障碍所致睡眠障碍大鼠海马超微结构的影响

Effect of Sinisan on ultra structure of hippo-campus in the intervene rats by post traumatic stress disorder

目的研究中药复方四逆散对创伤后应激障碍大鼠海马超微结构的影响。方法按照随机数表法将SD雄性大鼠随机分为5组,每组10只:空白对照组、模型组、阴性对照组、阳性对照组、实验组。除空白对照组外,其余4组动物均复制应激模型。模型组和空白对照组的大鼠饮食正常。于造模前1 h,阴性对照组的大鼠灌胃0.9%Na Cl;阳性对照组的大鼠灌胃盐酸帕罗西汀溶液4.2mg·kg^(-1);实验组的大鼠灌胃四逆散水煎液2.41 g·kg^(-1)。灌胃容积10m L·kg^(-1),每日灌胃1次,连续给药7 d。造模结束后,各组大鼠立即进行心脏灌注,采集海马组织,于30000倍电镜下观察并比较各组大鼠海马CA1、CA3区超微结构的差异性。结果空白对照组的海马CA1和CA3区的神经元细胞器丰富,线粒体圆形或长杆状,线粒体嵴结构清晰,粗面内质网呈条索样分布,核糖体丰富,高尔基复合体常见。模型组与空白对照组比较,细胞器明显损伤,细胞质结构空旷,线粒体肿胀,线粒体膜及嵴结构消失,粗面内质网池样扩张,提示幽闭电击对大鼠海马区神经元内细胞器产生明显损伤。阴性对照组与模型组比较,变化与其相似,提示0.9%Na Cl灌胃对大鼠没有明显的应激影响;而阳性对照组和实验组的海马区神经元细胞质内细胞器结构明显恢复,2组结构基本相似,提示盐酸帕罗西汀和四逆散均能明显改善PTSD大鼠海马区神经元细胞器结构。结论四逆散可以明显改善创伤后应激障碍大鼠海马CA1和CA3区神经元细胞器结构。

Objective To study the effect of Sinisan on the ultra structure of hippo - campus to the intervention in rats with post - traumatic stress disorder（PTSD）. Methods SD rats were divided equally into 5 groups,each group had ten rats：blank control group, model group,nega- tive control group, positive control group and experimental group. The blank control group did not copy the model, the normal feeding. Model group,repetitive post- traumatic stress disorder model was induced by current stimulation, but not treated. Negative control group, equal volume of 0. 9% NaCI. Positive control group （ paroxetine hydrochloride 0. 42 mg ·mL - 1 ） and experimentalgroup （ Sinisan, containing crude drug 0. 24 g · mL-l）. It given to drugs 1 h before the model establishment. The rats were administered with 10 mg·kg- 1, once a day, for a total of 7 d. In each group, rats was cardiac perfusion and the hippo -campus tissue was collected. The changes of ultra structure of CA1 and CA3 area in hippo - campus with rats were observed by trans- mission electron microscope. Results In the blank control group, the CA1 and CA3 neurons in the hippocampus were rich in cytoplasmic organelles, and the mitochondria were round or long, the structure of the mitochondrial cristae was clear, the rough endoplasmic reticulum was like a cord like distribution, and the ribosome was abundant. Compared with the blank control group, the organelle of CA1 and CA3 area in hippo - campus of the model group were significant- ly damaged, the cytoplasm was open, the mitochondria were swollen, the mitochondrial membrane and cristae disa- ppeared, and the rough endoplasmic reticulum was dilated. The results showed that the damage of the organelles in the hippocampal neurons of rats was induced by the electric shock. The model group was similar to the negative control group, which indicated that there was no significant effect on the stress of rats after intragastric administration. The in- tracellular organelles in the CA1 and CA3 neurons in the hippocampus of the positive control group and the experimen- tal group were significantly recovered, and the changes in the two groups were similar. These results suggest that both paroxetine hydroehloride and Sinisan can significantly improve the structure of CA1 and CA3 neurons in the hippoeam- pus of PTSD rats. Conclusion Snisan as traditional Chinese medicine compound can significantly improve the hippo- campus of rats with PTSD CA1 and CA3 neurons.