生菜资源表型性状变异及其与SSR标记的关联分析

Association Analysis Between Microsatellite Loci and Phenotypic Traits in Lactuca sativa L.

从世界范围内搜集的300多份生菜资源中筛选30份优质资源作为研究对象,调查12个表型性状变异,并进行SSR分子标记分析,探究表型性状与SSR标记之间的相关关系,以期为生菜分子标记辅助育种提供参考依据。结果表明:调查的30份资源表型性状均存在极显著差异,平均变异系数最大的为株幅(40.256%)。12个表型性状将30份生菜资源分成两大类,30份资源的遗传距离变化范围为0.083~0.667。14个多态性EST-SSR标记在30份生菜资源中扩增的条带数为3~8,HO和HE的平均值分别为0.156和0.680,PIC值变化范围为0.466~0.771。同样,14个标记将30份资源分成两大类,30份资源间的遗传距离为0.032~2.629,表型性状聚类结果与SSR标记聚类结果不相关。以单一性状聚类结果与SSR标记聚类结果进行相关性分析,大部分标记均与表型性状有相关性,且大部分引物组合表现为累积效应。

Among 300 lettuce germplasms collected worldwide,30 samples were selected as materials for the survey of phenotypic variation.And 12 traits were surveyed,SSR molecular markered analysis was conducted.Association analysis between microsatellite loci and phenotypic traits were performed to provide the theoretical basis for molecular marker assisted breeding of lettuce.The results showed that significant differences existed in phenotypic traits,and the remarkable one was strain amplitude（40.256%）.These phenotypic traits divided the 30 lettuce resources into two categories,with the genetic distance ranging between 0.083 and 0.667.Furthermore,each polymorphic EST-SSR marker amplified 3-8 alleles,and the mean HOand HE were 0.156 and 0.680,respectively.PIC value varied from 0.466 to 0.771.Similarly,the 14 SSR markers divided 30 resources into two categories,and genetic distance ranged from 0.032 to 2.629.The clustering results of phenotypic traits were not related to that of SSR markers.The correlation analysis between single trait and SSR marker showed that most markers were correlated with phenotypic traits.In particular,most marker combinations showed cumulative effects.