腹泻仔猪肠道沙门氏菌耐药性研究

Study on drug resistance of intestinal Salmonella in diarrhea piglets

为了解贵州省规模化养殖场腹泻仔猪肠道沙门菌耐药表型与耐药基因型相关性,本试验采集了贵州省7个规模化养猪场的腹泻仔猪肠道样本进行沙门菌分离鉴定,并对分离的沙门菌用药敏纸片法进行耐药表型分析,利用PCR方法对分离菌进行耐药基因扩增。结果显示：共分离鉴定沙门菌21株;药敏试验表明所鉴定的沙门菌对抗菌药物的耐药率从高到低依次是卡那霉素、磺胺异口恶唑和诺氟沙星等,且均为多重耐药株,耐药种类达8种以上;耐药基因与耐药表型分析结果显示分离的沙门菌β-内酰胺类耐药基因与耐药表型符合率高达100%,喹诺酮耐药基因与耐药表型符合率为62.5%,氨基糖苷耐药基因符合率为73.3%,磺胺类耐药基因符合率81.8%。研究结果对合理使用药物控制仔猪沙门菌感染提供了理论依据。

In order to understand the drug-resistance phenotype and genotype of Salmonella in diarrhea piglets of large-scale farms in Guizhou Province,the intestinal samples of diarrhea piglets in 6 large-scale pig farms in Guizhou Province were collected to isolate and identify Salmonella strains. Then the drug-resistance phenotype and genotype of these isolates were analyzed using the susceptibility test and PCR. In total,21 strains of Salmonella were isolated and identified from the samples. The drug-resistance test showed that the rates of resistance to drugs of the identified Salmonella were in the descending order of kanamycin,sulfamethoxazole and norfloxacin. All the isolates resisted against multiple antibiotics,and five of the isolates resisted against more than 8 different antibiotics. Furthermore,the results of the present study showed that the coincidence rate between β-lactam-resistance genotype and phenotype was as high as 100%. In the of quinolone resistance,the coincidence rate was 62. 5%, in aminoglycoside resistance, the coincidence rate was 73. 3%, and in sulfanilamide resistance,the coincidence rate was 81. 8%. The present study provides a theoretical support for rational use of drugs in controlling Salmonella infection in piglets.