桥本甲状腺炎合并甲状腺毒症对小鼠额叶谷氨酸含量的影响

Effect of hashimoto thyroiditis complicated by thyrotoxicosis on glutamate content in the frontal lobe of mice

目的研究桥本甲状腺炎合并甲状腺毒症对小鼠额叶谷氨酸含量和分布的影响。方法将30只CBA/J雌性小鼠分为3个组,即正常对照组、甲状腺功能正常的桥本甲状腺炎组(桥本组)、桥本甲状腺炎合并甲状腺毒症组(甲状腺毒症组)。腹腔注射猪甲状腺球蛋白建立甲状腺功能正常以及合并甲状腺毒症的桥本甲状腺炎小鼠模型。采用免疫组织化学方法观察各组小鼠额叶谷氨酸含量和分布情况。结果与正常对照组相比,甲状腺毒症组额叶Ⅱ~Ⅴ层中细胞内谷氨酸含量显著增高(P<0.01),桥本组Ⅱ、Ⅲ、Ⅴ层中细胞内谷氨酸含量增高(P<0.05);与桥本组相比,甲状腺毒症组额叶Ⅱ~Ⅴ层中细胞内谷氨酸含量增高(P<0.05)。结论桥本甲状腺炎会导致小鼠额叶细胞内谷氨酸含量增高,合并甲状腺毒症时增高更明显。

Objective To study the effect of Hashimoto thyroiditis complicated by thyrotoxicosis on glutamate con- tent and distribution in the frontal lobe of mice. Methods All 30 CBA/J female mice were divided into three groups, namely, normal control group, hashimoto thyroiditis group and hashimoto thyroiditis complicated by thyrotoxi- cosis group（ thyrotoxicosis group）. Both the hashimoto thyroiditis group and the thyrotoxicosis group were induced by intraperitoneal injection of porcine thyroglobulin. Immunohistochemical method was used to detect the glutamate content and distribution in the frontal lobe of mice. Results Compared to the normal controls, the intracellular glu- tamate content in the II ~ V layers of the frontal lobe of mice in the thyrotoxieosis group was singnificantly in- creased （P 〈 0. 01 ） and also increased in the II , II, V layers of the frontal lobe of hashimoto thyroiditis group mice （P 〈 0. 05 ）. In the thyrotoxicosis group, the intracellular glutamate content in the U ~ V layers of the frontal lobe was higher than the hashimoto thyroiditis group （P 〈 0. 05 ）. Conclusion The intracellular glutamate content in the frontal lobe of mice with hashimoto thyroiditis is higher than the normal controls, and this effect on mice with hashimoto thyroiditis complicated by thyrotoxicosis will be more obvious.