绣球菌多糖超声波修饰条件的响应面优化

Response Surface Optimization of Ultrasonic Modified Conditions of Polysaccharide from Sparassis latifolia

利用超声波对广叶绣球菌(Sparassis latifolia)多糖进行修饰,通过Box-Benhnken中心试验和响应面分析法,以料液浓度、超声功率和超声时间为自变量,绣球菌多糖的DPPH自由基清除率为响应值,对绣球菌多糖的超声波修饰工艺条件进行优化。优化得到超声波修饰工艺参数为:料液浓度为8 mg·mL^(-1),超声功率为550W,超声时间为60 min,该条件下绣球菌多糖(2 mg·m L-1)DPPH自由基清除率可达44.43%,较超声波修饰前提高了2.37倍。

In order to improve the antioxidant activity of polysaceharides from Sparassis latifolia, ultrasonic modification of the polysaccharides was investigated with Box-Benhnken center combination experiments and response surface methodology. The mathematical regression model is established about the dependent variable （DPPH· scavenging capacity） and independent vari- ables （concentration, ultrasonic power and treatment time）. The results showed that the optimum ultrasonic modified conditions were as follows： the concentration was 8 mg.mL-1, the ultrasonic power was 550 W, and the treatment time was 60 min. In this conditions the DPPtt. scavenging capacity of polysaccharides （2 mg.mL-l） was 44.43%, which was 2.37 times higher than that before ultrasonic modification.