杂交构树叶片组培快速繁殖体系的建立

Establishment of Tissue Culture and Rapid Propagation System of Hybrid Broussonetia papyrifera Leaves

为满足市场对杂交构树种苗的需求,以无菌苗叶片为外植体进行组织培养,优化其快速繁殖技术。结果表明:1)愈伤组织诱导及不定芽分化的适宜培养基为MS+6-BA 2.0mg/L+NAA 0.1mg/L,其愈伤组织诱导率达91.7%,不定芽分化率为88.3%。分化出的不定芽长势较好,叶片的颜色浓绿,且愈伤褐化、软化现象较少。2)继代增殖的适宜培养基为MS+6-BA 2.0mg/L+NAA 0.1 mg/L+GA30.5mg/L,增殖倍数可达5倍;3)生根适宜培养基为改良B5+6-BA 0.01mg/L+NAA 0.3mg/L+IBA0.5mg/L,生根率为60%。

The rapid propagation system of hybrid B.papyrifera seedlings was optimized by using B.papyriferaleaves as explants to meet the market demand of hybrid B.papyriferaseedlings.Results：1）The optimal medium for callus induction and adventitious bud differentiation is MS ＋ 2.0 mg/L 6-BA ＋0.1 mg/L NAA.The callus induction rate and adventitious bud differentiation rate can reach 91.7% and 88.3%respectively.The growth vigor and leaf color of the differentiated buds are strong and dark green.There is less in browning and softening of cali.2）The optimal medium for subculture multiplication of differentiated adventitious buds is MS＋2.0 mg/L 6-BA ＋0.1 mg/L NAA ＋0.5 mg/L GA3 and the multiplication times can be 5 times.3）The optimal medium for rooting of differentiated adventitious buds is B5 ＋ 0.01 mg/L 6-BA ＋ 0.3 mg/L NAA ＋0.5 mg/L IBA and the rooting rate of differentiated adventitious buds is up to 60%.