沙眼衣原体增强HPV持续感染免疫机制的研究

Study on the Immune Mechanism of Chlamydia Trachomatis in Enhancing HPV Persistent Infection

目的研究沙眼衣原体(Chlamydia trachomatis,Ct),增强人乳头状瘤病毒(Papillomavirus,HPV)持续感染的免疫机制。方法选取静安区闸北中心医院2016年7月—2017年7月收治的100例HPV感染患者,20例正常人做对照组,根据其是否伴有Ct感染将其分为Ct/HPV共感染组和HPV单纯感染组,比较不同组别患者的混合淋巴细胞抗原递呈能力,LC密度,细胞因子分泌情况。结果 Ct/HPV共感染组和HPV单纯感染组的细胞因子分泌情况均与正常对照组相比存在显著差异(P<0.05),且Ct/HPV共感染组的IL-1β、IL-6、IL-10和IL-12均明显高于HPV单纯感染组,IFN-γ和TNF-α显著低于HPV单纯感染组(P<0.05);Ct/HPV共感染组和HPV单纯感染组的抗原递呈能力与对照组相比均呈下降趋势(P<0.05),且Ct/HPV共感染组抗原递呈能力显著低于HPV单纯感染组患者(P<0.05);LC密度在HPV单纯感染组患者出现显著降低显著低于对照组(P<0.05),而Ct/HPV共感染组患者的LC密度与HPV单纯感染组存在显著差异(P<0.05)。结论 Ct感染改变细胞因子分泌,并通过减少抗原递呈细胞,抑制细胞介导的免疫阻止了机体对HPV的清除,引起HPV的持续感染,而加快诱发宫颈癌的进程。

OBJECTIVE To study the immune mechanism of Chlamydia trachomatis（Ct） to enhance the persistent infection of human papillomavirus （HPV）. METHODS It collect 100 cases of patients with HPV infection from July 2016 to July 2017, and 20 normal cas- es as control group, according to whether the patients with Ct infection or not, cases were divided into Ct/HPV infection group and simple HPV infection group. It compare the different groups of patients with mixed lymphocyte antigen presenting ability, LC density, eytokine secretion. RESULTS CffHPV coinfection group and simple HPV infection group cytokine secretion were of significant differ- ence （P〈 0.05）compared with control group, and IL-1, IL-6, IL-10 and beta IL-12 in Ct/HPV group were significantly higher than that of HPV simple infection group, IFN-γand TNF-α were significantly lower than that of HPV infection group （P〈0.05）; antigen presenting ability of Ct/HPV co infection group and simple HPV infection group decreased when compared with the control group （P〈 0.05）, and antigen presenting ability of Ct/HPV group was significantly lower than that of HPV simple infection group （P〈0.05）; the density of LC in HPV infected patients significantly decreased than the control group （P〈0.05）, and the density of LC in Ct/HPV co infection were significantly lower than that of HPV group （P〈0.05）. CONCLUSION Ct infection change the cytokine secretion, reduce antigen presenting cells, inhibit cell mediated immunity, cause HPV persistent infection, and thus accelerate the process of inducing cervical cancer.