纤维素酶制备香蕉皮纳米纤维素的工艺条件研究

Study on the process conditions of preparation of nano-cellulose in banana peel by cellulase

采用纤维素酶法制备香蕉皮纳米纤维素。研究酶解时间、纤维素酶浓度、酶解温度3个因素对香蕉皮纳米纤维素产率的影响,通过响应曲面分析方法优化其酶解工艺,得到纤维素酶法制备香蕉皮纳米纤维素的最佳合适的工艺条件。结果表明:最佳条件为温度60℃,酶浓度200μ/mL,酶解时间180min,产率为53.08%。

Nano-cellulose in banana peel was prepared by cellulase. The effects of enzymolysis time,cellulase concentration and enzymolysis temperature on the yield of nano-cellulose in banana peel were studied. The process of enzymatic hydrolysis was optimized by the response surface analysis method,and the optimum process conditions for the production of nano-cellulose by cellulose enzyme were obtained. The results showed that the optimal conditions were ：temperature was 60 ℃,enzyme concentration was 200 u/mL,enzymolysis time was 180 min,and the yield was 53.08%.