基于氮掺杂碳量子点构建荧光传感器对肝素选择性测定

Selective determination of heparin based on fluorescence sensors constructed from nitrogen doped carbon quantum dots

将柠檬酸置于单乙醇胺中,通过简单加热实现快速、大规模的合成氮掺杂荧光碳点。所得氮掺杂碳量子点被370 nm的光激发后在458 nm处有较强的荧光发射,最大吸收波长为315 nm。肝素能增强该碳量子点的荧光且具有线性关系。基于该现象,设计了一种以氮掺杂碳量子点为荧光传感器检测肝素的方法。在最佳实验条件下,该方法的线性范围为0.13~2.63 U/mL,检出限为0.01 U/mL。实际样品加标回收率在93.9%~101.2%之间。

Rapid and large-scale synthesis of NCDs was accomplished by sample heating of the precursor mixture of citric acid and monoethanolamine.Opon excited by 370 nm,NCDs exhibit a strong fluorescence emission at 458 nm,and the maximum absorption wavelength is 315 nm.The fluorescence of NCDs can be enhanced by heparin in a linear relationship with heparin concentration.Based on this,an analytical method for the detection of heparin with NCDs as fluorescence sensors was designed.Under the optimum experimental conditions,the linear detection range of this method is 0.13-2.63 U/m L,and the detection limit is 0.01 U/m L.The recovery rate of the actual sample is between 93.9% - 101.2%.