鹿茸血提取物改变乳腺癌荷瘤小鼠免疫细胞比例并抑制移植瘤生长

Blood extracts from pilose antler changes the proportion of immune cells in mammary tumor-bearing mice and inhibit the growth of transplanted tumor

目的研究鹿茸血对健康及荷瘤小鼠免疫系统的影响。方法建立荷乳腺癌小鼠模型,流式细胞术检测鹿茸血灌胃处理后,健康鼠以及荷瘤小鼠外周血总T细胞(CD3^+)、辅助性T细胞(CD4^+)、毒性T细胞(CD8^+)、总B细胞(CD45R^+)以及髓性抑制细胞(MDSC,CD11b^+Gr-1^+)比例。结果鹿茸血提取物能下调健康小鼠外周血总T细胞,减弱细胞免疫,上调B细胞比例,增强体液免疫,并且能够增加荷瘤小鼠辅助性T细胞、毒性T细胞、总T细胞以及总B细胞比例,并降低MDSC比例,抑制移植瘤的生长速度。结论鹿茸血天然提取物增强小鼠免疫功能,抑制移植瘤生长。

Objective To study the influence of blood extracts from pilose antler on the immune system in healthy mice and tumor-bearing mice. Methods A mouse mammary tumor transplantation model was established and both the healthy and the tumor-bearing mice were gavaged with the blood extracts of pilose antler. The percentages of mouse peripheral blood CD3~＋T cells,CD4~＋T cells,CD8~＋T cells,B220~＋B cells and myeloid-derived suppressor cells（ MDSCs） in the healthy and tumor-bearing mice were analyzed by flow cytometry. Results The blood extracts of pilose antler quantitatively up-regulated the helper T（ CD4~＋） cell numbers,elevated the total T cell and B cell numbers,reduced the MDSC number and inhibited tumor growth. Conclusion The blood extracts of pilose antler can enhance mouse immune functions and inhibit transplanted tumor growth.