结直肠癌患者人类端粒酶反转录酶mRNA的表达及影响因素

Expression of human telomerase reverse transcriptase mRNA in colorectal cancer patients and its clinical significance

目的观察结直肠癌患者人类端粒酶反转录酶（hTERT） mRNA的表达，并分析其影响因素。方法分析在我院接受治疗的80例结直肠癌患者，同时选取80例健康成年人的临床资料作为对照。观察两组患者结直肠组织hTERT mRNA表达，比较不同临床病理特征的结直肠癌患者结直肠组织hTERT mRNA的表达，分析影响结直肠癌患者hTERT mRNA表达的影响因素。结果结直肠癌患者的结直肠组织hTERT mRNA阳性表达率为87.50%，明显高于正常对照组（6.25%）；有腹腔淋巴结转移、组织学分型为管腺癌、TNM分期为Ⅲ＋Ⅳ期、分化程度为中低分化的结直肠癌患者其结直肠组织hTERT mRNA阳性表达率较高（χ2＝4.068、4.898、8.447、6.154；P＝0.044、0.027、0.004、0.013），而不同年龄、性别、肿瘤部位和大小的结直肠癌患者结直肠组织hTERT mRNA阳性表达率比较，差异无统计学意义（χ2＝2.593、0.258、0.878、0.152、1.755；P＝0.107、0.612、0.349、0.696、0.185）；有无腹腔淋巴结转移、组织学分型是影响结直肠癌患者结直肠组织hTERT mRNA阳性表达率的危险因素，其比值比（OR）分别为4.115和3.985。结论结直肠癌患者结直肠组织hTERT mRNA的阳性表达率较高，且与患者是否存在淋巴结转移、肿瘤分期和组织分型密切相关。

Objective To investigate the expression of human telomerase reverse transcriptase （hTERT） mRNA in colorectal cancer patients, and to explore its clinical significance.Methods The clinical data of 80 patients with colorectal cancer who received treatment in our hospital from February 2014 to August 2015 were retrospectively analyzed. At the same time, the clinical data of 80 healthy adults were selected as control. To observe the expression of hTERT mRNA in colorectal tissue of two groups of patients, and to compare the expression of hTERT mRNA in colorectal tissue of colorectal cancer patients with different clinicopathological features, and to analyze the factors influencing the expression of hTERT mRNA in colorectal cancer patients.Results The positive expression rate of hTERT mRNA in colorectal tissue of colorectal cancer patients was 87.50%, which was significantly higher than that in normal control group （6.25%）. Abdominal lymph node metastasis, histological type and tube adenocarcinoma and TNM stage Ⅲ＋ Ⅳ, the degree of differentiation for poorly differentiated colorectal cancer colorectal tissue the positive expression rate of hTERT mRNA was higher （χ2=4.068, 4.898, 8.447, 6.154; P=0.044, 0.027, 0.004, 0.013）, different age, gender, colorectal tissues of patients with colorectal cancer hTERT mRNA expression of tumor size and location was not significant difference （χ2=2.593, 0.258, 0.878, 0.152, 1.755; P=0.107, 0.612, 0.349, 0.696, 0.185）. Abdominal lymph node metastasis and histological type were the risk factors of hTERT mRNA positive rate in patients with colorectal cancer. The odds ratio （OR） values were 4.115 and 3.985, respectively.Conclusion The expression of hTERT mRNA in colorectal tissue of patients with colorectal cancer was higher, and the existence of patients with lymph node metastasis, tumor stage and histological type are closely related, as an important index for clinical screening of colorectal cancer.