带鱼肠道中芽孢杆菌的分离鉴定及其发酵液抗菌性质研究

Isolation and Identification of Bacillus from Gastrointestinal Tract of Hairtail(Trichiurus haumela) and Antibacterial Characteristics of Their Fermented Liquids

从带鱼肠道中分离筛选到1株具有广谱抗菌活性的菌株JFL15,其发酵液对嗜水气单胞菌、美人鱼发光杆菌、哈维氏弧菌、溶藻弧菌、大肠杆菌、迟钝爱德华氏菌、铜绿假单胞菌、副溶血弧菌、创伤弧菌、查氏弧菌均有较强抑菌作用。通过形态特征、生理生化特征、16S rDNA序列分析以及系统发育进化分析,菌株JFL15与暹罗芽孢杆菌的同源性最高,达98.05%,初步鉴定为暹罗芽孢杆菌。对暹罗芽孢杆菌JFL15的发酵液进行研究发现,暹罗芽孢杆菌JFL15的发酵液对酶、温度、酸碱度和紫外线均不敏感,且发酵产物储存较长时间后仍具有较强抑菌活性。

A strain with broad-spectrum antimicrobial activity,named JFL15,was isolated from the gastrointestinal tract of hairtail（Trichiurus haumela）in Guangzhou,China,whose fermented liquids showed strong antimicrobial activities against 15 pathogens in aquaculture including Aeromonas hydrophila,Escherichia coli,Edwardsiella tarda,Pseudomonas aeruginosa,Photobacterium damsela,Vibrio alginolyticus,V.harveyi,V.parahaemolyticus,V.vulnificus and V.chagasii.The strain JFL15 was identified as Bacillus siamensis base on the morphological,and physiological biochemical characteristics together with similarity analysis of 16S rDNA sequences.The fermentation liquor of strain JFL15 was found to be stable to enzymatic hydrolysis,high temperature（up to 100℃）,pH range from 3 to 9 and ultraviolet radiation,and the antimicrobial activity was maintained in the liquor stored for 28 days at 4 ℃.