外加CO_2对盐藻生长及碳酸酐酶活性的影响

Effect of Extra CO_2 on Growth and Activity of Carbonic Anhydrase of Alga Dunaliella salina

在光照阶段开始前向盐藻培养体系中通入CO_2至其饱和(pH=6.0),探究外加CO_2对盐藻生长及细胞碳酸酐酶活性的影响。结果显示,未加CO_2的对照组盐藻培养液的pH在8.10~8.20,外加CO_2至饱和时培养液的pH从6.00升至7.04,盐藻生物量、叶绿素含量、细胞内外碳酸酐酶比活性均显著高于未加CO_2的培养体系;未加CO_2和通入CO_2的两种体系中细胞外碳酸酐酶比活性均显著高于细胞内碳酸酐酶比活性,前者分别是后者的1.39、1.71倍。这表明在盐藻培养体系中通入CO_2至饱和时,盐藻通过提高细胞内外的碳酸酐酶活性加快对CO_2的吸收,进而快速生长,提高生物量。此结果为利用盐藻吸收转化工业排放的CO_2并获取盐藻生物量的进一步研究提供了试验依据。

Extra CO_2 was bubbled to saturate（pH=6.0）in culture medium of algaDunaliella salina at the beginning of illuminating period,and the effects of extra CO_2 on the growth and the activity of carbonic anhydrase（CA）were studied in the alga.The results showed that pH in the medium was held at 8.10—8.20 during the culture period without additional CO_2,while pH gradually increased to 7.04 from 6.00 with additional CO_2.Meanwhile,there were more biomass,chlorophyll content,and activities of intracellular and extracellular CAs in the medium with CO_2 addition than those in the medium without additional CO_2.Extracellular CA activity was significantly higher than intracellular CA activity in both media,0.39 times higher in the medium without additional CO_2,and 0.71 times higher with additional CO_2.These findings indicated that the algaD.salina had good growth by increasing the activities of intracellular and extracellular CAs to absorb more CO_2 in the medium with saturated CO_2,which will provide experimental basis for further understanding of absorption and transform CO_2 emitted from industry by D.salina.