芦荟对感染根管内厌氧菌抑菌作用的体外研究

A Study on the Antibacterial Activities of the Aloe on Anaerobic Strains from the Infected Root Canal in vitro

目的:研究中药芦荟对根管内感染的5种厌氧菌的体外抑菌作用。方法:取感染根管内的常见优势菌:牙髓卟啉单胞菌(Porphyromonas endodontalis,P.e)、大肠杆菌(Escherichia coli,E.coli)、金黄色葡萄球菌(Staphylococcus aureus,S.a)、链球菌(Streptococcus)及中间普雷沃菌(Pseudomonas,P.i)的菌株,采用试管二倍稀释法,检测不同浓度芦荟制剂对上述菌株的抑菌效果。设空白对照组,阳性对照组为常规使用的根管治疗的封闭材料—氢氧化钙。采用纸片扩散法检测芦荟制剂的抑菌环大小。结果:不同浓度芦荟制剂均能明显抑制5种实验菌株生长,5种菌株的最小抑菌浓度(MIC)与最低杀菌浓度(MBC)不同,芦荟制剂对上述5种细菌在实验浓度范围内与氢氧化钙抑菌效果无显著差异;甲基红染色发现,不同MIC浓度的芦荟制剂使大肠杆菌变为淡红色,而使其他4种菌株均呈黄色;不同MIC浓度的芦荟制剂使5种菌株均产生抑菌环。结论:芦荟制剂抑菌效果较明显,可能作为根管内封药物的候选药物。

Objective： To research the antibacterial activities of the aqueous extract of Chinese aloe on five kinds of anaerobic bacteria from the infected root canal in vitro. Methods： Porphyromonas endodontalis,（P. e）, Escherichia coli（E. coli）, Staphylococcus aureus（S. a）, Streptococcus and Pseudomonas（P. i） were selected as the common dominant bacteria in the root canal. The antibacterial effect of different concentrations of aloe suppository on the above strains was tested by double dilution method. The routine use of root canal drug,Calcium hydroxide was used as a control. The anti-bacterical ring size of aloe vera was measured by the paper diffusion method. Results：Different concentrations of the aqueous extract of Aloe could significantly inhibit the growth of five kinds of experimental strains. The values of minimal inhibitory and bactericidal concentrations（MIC and MBC） are different among the strains above. There was no significant difference between the experimental concentrations of aloe vera and calcium hydroxide. The results of methyl red experiment showed that aloe preparation in different concentrations of MIC turned the E. coli into a reddish color, while the other four strains became yellow. The aloe vera preparations with different MIC concentrations allowed the five strains to produce antibacterial rings. Conclusions：The antibacterial effect of aloe vera is obvious, which may serve as a candidate drug for the drug encapsulation.