喘息型支气管炎患儿外周血中25-(OH)D_3与IgE浓度水平变化及其发病机制分析

Analysis on the changes of 25-(OH)D_3 and IgE concentrations in peripheral blood of children with asthmatic bronchitis and the pathogenesis

目的分析喘息型支气管炎患儿外周血中25-(OH)D_3与IgE浓度水平变化及发病机制。方法选取2015年1月-2017年1月在义乌市妇幼保健院接受治疗的确诊为喘息型支气管炎的患儿70例为喘息组;选取同期在院治疗的确诊为支气管肺炎的患儿70例为肺炎组;在选取同一时间在院接受体检的健康幼儿70例为对照组。使用ELISA方法检测3组对象血清IgE和25-(OH)D_3含量,根据试剂盒说明书进行相关操作。结果 3组对象血清内25-(OH)D_3含量变化情况:喘息组与对照组、肺炎组比较,25-(OH)D_3含量下降,差异均有统计学意义(均P<0.05);肺炎组与对照组比较,血清内25-(OH)D_3含量下降,差异有统计学意义(P<0.05);3组对象血清内IgE含量变化情况:喘息组与对照组、肺炎组比较,其血清内IgE含量上升,差异均有统计学意义(P<0.05);肺炎组与对照组比较,其血清内IgE含量上升,但差异无统计学意义(P>0.05);喘息组患儿血清内25-(OH)D_3含量与IgE呈负相关(r=-0.184,P<0.05);肺炎组患儿血清内25-(OH)D_3含量与IgE呈负相关(r=-0.374,P<0.05);对照组血清内25-(OH)D_3含量和IgE无相关性(P>0.05)。结论喘息性疾病的发生与血清内维生素D含量下降有关,机体内缺乏维生素D会刺激IgE含量的增多,从而导致喘息性疾病的发生。

Objective To analyze the changes of 25-（OH）D3 and IgE concentrations in peripheral blood of children with asthmatic bronchitis and the pathogenesis. Methods A total of 70 children with asthmatic bronchitis were selected from Yiwu Maternal and Child Health Care Hospital from January 2015 to January 2017 as asthma group. Seventy children diagnosed as bronchopneumonia definitely in the hospital during the same period were selected as pneumonia group. Seventy healthy children receiving physical examination in the hospital during the same period were selected as control group. ELISA was used to detect the concentrations of serum IgE and 25-（OH）D3 in the three groups. The relevant operation was carried out according to kit instructions. Results The concentration of serum 25-（OH）D3 in asthma group was statistically significantly lower than those in control group and pneumonia group（ P0. 05）. The concentration of serum25-（OH）D3 in pneumonia group was statistically significantly lower than that in control group（ P0. 05）. The concentration of serum IgE in asthma group was statistically significantly higher than those in control group and pneumonia group（ P0. 05）. The concentration of serum IgE in pneumonia group was higher than that in control group,but there was no statistically significant difference（ P〈0. 05）. In asthma group,serum 25-（OH）D3 concentration was negatively correlated with serum IgE concentration（ r =-0. 184,P〈0. 05）. In pneumonia group,serum 25-（OH）D3 concentration was negatively correlated with serum IgE concentration（ r =-0. 374,P0. 05）. In control group,serum 25-（OH）D3 concentration was not correlated with serum IgE concentration（ P0. 05）. Conclusion The occurrence of wheezing diseases is related to the decrease of vitamin D content in serum. The lack of vitamin D will stimulate the increase of IgE content,which will lead to wheezing disease.