牛津杯法测定抗菌肽对四种有害微生物的抑制效果

The inhibitory effect of antimicrobial peptide against four kinds of harmful microorganisms by the Oxford cup method

试验采用牛津杯法测定抗菌肽对金黄色葡萄球菌(Staphylococcus aureus)、溶藻弧菌(Vibrio alginolyticus)、副溶血弧菌(Vibrio parahaemolyticus)、嗜水气单胞菌(Aeromonas hydrophila)有害菌的抑菌效果。结果表明:抗菌肽对四种有害菌均有一定的抑菌效果,对金黄色葡萄球菌(3×1016CFU/ml)的抑菌效果最好,其次是副溶血弧菌(1.6×10^(14)CFU/ml)、溶藻弧菌(7.5×10~5CFU/ml),对嗜水气单胞菌(8.5×10~5CFU/ml)的抑菌效果最弱。抗菌肽对四种有害菌的最低抑菌质量浓度(MIC)分别是0.8、0.8、1.6、3.2 mg/ml。

In this experiment, the Oxford cup method were performed to evaluate the antibacterial peptide antibacterial effect on harmful bacteria such as Staphylococcus aureus, Vibrio alglnolyticus, Vibrio parahaemolyticus and Aeromonas bydrophila. The results showed that antimicrobial pep- tide had certain inhibitory effect on four kinds of detrimental bacteria, the antimicrobial effect was the best against Staphylococcus aureus （3×10^6 CFU/ml ）, followed by Vibrio parahaemolytieus （1.6×10^14 CFU/ml）, Vibrio alginolytieus（ 7.5×10^5 CFU/ml）, the antibaeterial effeet on Aeromonas hydrophila （8.5×10^5 CFU/ml）was the weakest. The minimum inhibitory concentration （MIC） of antimierobial peptide against four kinds of harmful bac- teria were 0.8, 0.8, 1.6, 3.2 mg/ml, respeetively.