纤维支气管镜检标本微小RNA-21在非小细胞肺癌诊断中的临床应用价值

Clinical value of microRNA-21 level in the diagnosis of non-small cell lung cancer by fiber-optic bronchoscopy

目的探讨纤维支气管镜检标本微小RNA-21(mi RNA-21)在非小细胞肺癌(NSCLC)诊断中的临床应用价值。方法选取80例NSCLC患者作为NSCLC组,选取同期接受镜检筛查的80例肺炎或肺结核患者作为对照组。检测两组患者的镜检物mi RNA-21表达水平,比较两组患者的细胞因子和肿瘤标志物水平,分析NSCLC患者镜检物mi RNA-21水平与细胞因子和肿瘤标志物水平的相关性。结果 NSCLC组患者的镜检物mi RNA-21水平为(8.29±1.15),明显高于对照组的(1.35±0.41),差异有统计学意义(P﹤0.001)。NSCLC组患者的γ-干扰素(IFN-γ)、白细胞介素-1β(IL-1β)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平明显高于对照组,差异均有统计学意义(P﹤0.001)。NSCLC组患者的癌胚抗原(CEA)、神经元特异烯醇化酶(NSE)和角蛋白CYFRA21-1水平明显高于对照组,差异均有统计学意义(P﹤0.001)。NSCLC患者的镜检物mi RNA-21表达水平与细胞因子IFN-γ、IL-1β、IL-4、IL-6、TNF-α和肿瘤标志物CEA、NSE、CYFRA21-1水平均呈正相关(P﹤0.05)。结论 NSCLC患者纤维支气管镜检标本中mi RNA-21的表达水平较高,且与细胞因子和肿瘤标志物水平密切相关。

Objective To observe the expression of micro RNA-21（mi RNA-21） in patients with non-small cell lung cancer（NSCLC） and to analyze its clinical significance. Method 80 patients with NSCLC were included as NSCLC group, meanwhile, another 80 cases with pneumonitis or tuberculosis who underwent fiber-optic bronchoscopy were recruited as control group. The level of mi RNA-21 in the two groups was observed microscopically, the level of cytokines and tumor markers of the two groups were compared, and then the correlation between mi RNA-21 level and cytokines and tumor markers in NSCLC patients was analyzed. Result The level of mi RNA-21 in the NSCLC group was（8.29±1.15）, which was significantly higher than that of the control group at（1.35±0.41）（P〈0.001）. The levels of IFN-γ, IL-1β,IL-4, IL-6 and TNF-α in NSCLC group were higher than those in the control group, with significant difference observed（P〈0.001）. The levels of CEA, NSE and CYFRA21-1 in NSCLC group were higher than those in the control group, demonstrating statistically significant difference（P〈0.001）. The level of mi RNA-21 in the patients with NSCLC was positively correlated with the level of cytokines which were IFN-γ、IL-1β、IL-4、IL-6、TNF-α and tumor markers of CEA, NSE,CYFRA21-1（P〈0.05）. Conclusion The mi RNA-21 in the specimen of NSCLC patients with fiber-optic bronchoscopy is highly expressed, and is closely related to the level of cytokines and tumor markers.