红花-甘草配伍对寒凝血瘀大鼠肠系膜动脉血管舒缩功能的影响

Effect of safflower-liquorice on vasomotion of mesenteric artery in rats with cold coagulation and blood stasis

目的:研究红花-甘草配伍对寒凝血瘀证大鼠肠系膜动脉血管舒缩功能的影响。方法:采用冰水应激大鼠30d制作寒凝血瘀证大鼠模型,应用生物机能实验系统测定大鼠肠系膜动脉血管收缩力。结果:与对照组比较,模型组苯肾上腺素(PE)收缩血管的量效曲线、β2受体拮抗剂ICI 118551预处理后肾上腺素(AD)收缩血管的量效曲线及沙丁胺醇(SAL)舒张血管的量效曲线均明显右移,最大收缩幅度(E_(max))或舒张幅度(R_(max))均明显降低,pD_2值均明显减小;与模型组比较,红花组和红花-甘草组此三种量效曲线明显左移,E_(max)或R_(max)明显升高,红花-甘草组的作用强于红花组。用高K^+和PE收缩血管,Ach对模型组大鼠血管的内皮依赖性舒张作用明显减弱,红花组和红花-甘草组Ach舒张血管的作用明显增强,红花-甘草组的作用强于红花组。结论:红花-甘草配伍能明显改善寒凝血瘀证大鼠肠系膜血管的收缩和舒张功能,其机制可能与调节肾上腺素受体和保护血管内皮功能有关。

OBJECTIVE To investigate effect of safflower-liquorice on vasomotion of mesenteric artery in rats with cold coagulation and blood stasis induced by cold stress.METHODS Cold coagulation and blood stasis model rats were induced by cold stress for 30 days.Adrenergic receptor-mediated vosoactivity and endothelium-dependent relaxation on rat mesenteric artery ring segments were monitored by a myograph system.RESULTS Compared with control arterial segments,the contractile response curves of phenylephrine（PE）and adrenaline（AD）with ICI 118551 as well as the relaxation response curve of salbutamol（SAL）in the arterial segments from model rats were all shifted to right.The decreased maximum contraction（Emax）induced by PE and AD with ICI 118551 as well as the decreased maximum relaxation（Rmax）of SAL was seen in the arterial segments from model rats.After oral administration of aqueous extracts of safflower and safflower-liquorice,the contractile response curves of PE and AD with ICI 118551 as well as the relaxation response curve of SAL in the mesenteric arterial segments were all shifted to left.The relaxation in endothelium-intact arterial segments induced by acetylcholine（Ach）was significantly decreased in model rats,and aqueous extracts of safflower and safflower-liquorice significantly attenuated the decreased relaxation in endothelium-intact arterial segments induced by Ach.The effect of aqueous extract of safflower-licorice on vasomotion of mesenteric artery in rats with cold coagulation and blood stasis was stronger than that of aqueous extract of safflower.CONCLUSION Safflower and safflower combined with liquorice exhibit better efficacy against cold coagulation and blood stasis syndrome by regulating alpha and beta adrenergic receptors and protecting endothelium of mesenteric artery in rats with cold coagulation and blood stasis.