食管鳞癌组织中GSK3β表达的临床意义

Clinical significance of GSK3β expression in esophageal squamous cell carcinoma tissue

目的:分析GSK3β在食管鳞癌组织中的表达及临床意义。方法:采用实时荧光定量PCR、Western blot、免疫组化等方法检测GSK3β在52例食管鳞癌及相应癌旁组织中的表达,并采用Cox回归分析GSK3β表达与食管鳞癌患者术后预后的关系。结果:食管鳞癌组织中GSK3βmRNA及蛋白表达水平、阳性表达率均高于相应癌旁组织(P<0.05)。Cox回归分析表明GSK3β为食管鳞癌患者术后预后的独立危险因素(β=1.095,P=0.026)。结论:GSK3β表达可能促进了食管鳞癌的发生发展;GSK3β可能成为食管鳞癌靶向治疗的分子靶点之一。

Aim： To characterize the expression level of GSK3β and its clinical significance in esophageal squamous cell carcinoma（ ESCC） tissue. Methods： The mRNA and protein expression levels of GSK3β in 52 cases of ESCC and matched adjacent tissue samples were determined by Real-time PCR,Western blot and immunohistochemistry,respectively,and the correlation between the expression of GSK3β and the prognosis of patients was also analyzed using Cox regression. Results： The mRNA and protein expression levels,and the positive rate of GSK3β in ESCC tissue were higher compared with those in adjacent noncancerous mucosa tissue（ P〈0. 05）. Cox regression analysis showed that GSK3β was an independent risk factor for the prognosis of patients with ESCC（ β = 1. 095,P = 0. 026）. Conclusion： GSK3β expression may promote the development of ESCC,and GSK3β may be one of the molecular targets for treatment of ESCC.