LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响

Effect of LncRNA MEG3 on proliferation,invasion and apoptosis in osteosarcoma cells

目的:探讨LncRNA MEG3(MEG3)对骨肉瘤细胞增殖、侵袭和凋亡的影响。方法:构建pc DNA3.0-MEG3重组质粒。qRT-PCR法检测MEG3在人正常成骨细胞h FOB1.19,转染pc DNA3.0-MEG3前后人骨肉瘤细胞系MG63、U-2 OS中的表达,以转染空质粒和未转染的骨肉瘤细胞作对照。采用CCK-8法检测转染空质粒和pc DNA 3.0-MEG3的MG63、U-2 OS细胞的增殖能力,采用Transwell迁移实验和流式细胞术分别检测细胞迁移能力和细胞凋亡。结果:与正常成骨细胞相比,MEG3在骨肉瘤细胞中的表达降低(P<0.05)。转染pc DNA3.0-MEG3重组质粒后MG63和U-2 OS细胞的增殖和侵袭能力减弱,凋亡增强(P<0.05)。结论:MEG3在骨肉瘤细胞中低表达;MEG3过表达可抑制骨肉瘤细胞增殖和侵袭,促进凋亡。

Aim： To investigate the effect of LncRNA MEG3（ MEG3） on the proliferation,invasion and apoptosis of osteosarcoma cells. Methods： The expression of MEG3 in normal human osteoblast h FOB1. 19 and human osteosarcoma cell line MG63 and U-2 OS were detected by qRT-PCR. We constructed the recombinant plasmid of pc DNA3. 0-MEG3.Then the empty plasmid and the recombinant plasmids were transfected into human osteosarcoma cell lines,the expression of MEG3 in MG63 and U-2 OS was detected by qRT-PCR,and cell proliferation,migration and apoptosis were detected by CCK-8 assay,transwell migration assay and flow cytometry assay,respectively. Results： Compared with h FOB1. 19,the expressions of MEG3 in osteosarcoma cells were significantly decreased（ P〈0. 05）. The abilities of proliferation and invasion of MG63 and U-2 OS cells transfected with pcD NA3. 0-MEG3 were significantly reduced,and the cell apoptosis was significantly increased（ P〈0. 05）. Conclusion： MEG3 is downregulated in osteosarcoma cells,and the overexpression of MEG3 can inhibit the proliferation and invasion,and promote apoptosis of osteosarcoma cells.