肠毒素脆弱拟杆菌具核梭杆菌和幽门螺旋杆菌在肠道内富集度与结直肠癌的关系

Relationships between the enrichment of ETBF, Fn, Hp in intestinal and colorectal cancer

目的探究肠毒素脆弱拟杆菌（ETBF）、具核梭杆菌（Fn）、幽门螺旋杆菌（Hp）在粪便和组织中的富集度与结直肠癌的关系。方法收集24例结直肠癌、31例腺瘤性息肉患者的粪便、病变组织、病变旁组织，收集20例正常体检者的粪便和组织，采用实时荧光定量聚合酶链反应（RT-qPCR）方法检测ETBF、Fn、Hp的拷贝数，采用免疫组化方法检测结直肠癌组、正常对照组黏膜组织中表皮生长因子受体（EGFR）、p53的表达，分析EGFR、p53表达强度与3种细菌的关系。结果粪便中，ETBF、Fn拷贝数表现为结直肠癌组〉腺瘤性息肉组〉正常对照组（P〈0.05），Hp拷贝数表现为结直肠癌组〉正常对照组（P〈0.01），腺瘤性息肉组〉正常对照组（P〈0.01）。组织中，ETBF、Fn拷贝数表现为结直肠癌组〉腺瘤性息肉组〉正常对照组（P〈0.05），Hp拷贝数表现为结直肠癌组〉正常对照组（P〈0.01），腺瘤性息肉组〉正常对照组（P〈0.01）。腺瘤性息肉组、结直肠癌组的病变组织与病变旁组织中3种细菌的拷贝数差异均无统计学意义（P〉0.05）。结直肠癌组EGFR表达的阳性率为83.33%（20/24），p53表达的阳性率为66.67%（16/24）。EGFR、p53表达强度分别为阴性、弱阳性、中度阳性、强阳性的结直肠癌3种细菌的拷贝数差异无统计学意义（P〉0.05）。结论ETBF、Fn、Hp在结直肠癌、腺瘤性息肉患者粪便和组织中的富集度高，可能与结直肠癌、腺瘤性息肉的发生有关。临床试验注册 中国临床试验注册中心，ChiCTR-BOC-17012509。

ObjectiveTo explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer.MethodsFeces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis （ETBF）, Fusobacterium nucleatum （Fn） and Helicobacter pylori （Hp） were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias.ResultsIn the feces, copy numbers of ETBF and Fn were as follous： colorectal cancer group〉adenomas group〉healthy control group （P〈0.05）. Copy numbers of Hp were as follous： colorectal cancer group〉healthy control group （P〈0.01）; adenomas group〉healthy control group （P〈0.01）. In the tissue, copy numbers of ETBF, Fn were as follows： colorectal cancer group〉adenomas group〉healthy control group （P〈0.05）. Copy numbers of Hp were as follows： colorectal cancer group〉healthy control group （P〈0.01）; adenomas group〉healthy control group （P〈0.01）. Copy numbers of those three bacteria in the lesion tissue and the adjacent tissue had no significant difference. This happened both in colorectal cancer group and adenomas group. The different expression intensity of EGFR, p53 and the number of three bacteria showed no obviously statistical correlation（P〉0.05）.ConclusionAdenomatous polyp and colorectal cancer patients show high enrichment of ETBF, Fn and Hp in both feces and tissues. ETBF, Fn and Hp probably contribute to the development of adenomatous polyp and colorectal cancer. Trial registration Chinese Clinical Trial Registry, ChiCTR-BOC-17012509.