FPIA和HPLC法测定肾移植病人环孢素浓度比值的临床意义

The clinical significance of the cyclosporine concentration ratio in the patients of renal transplantation determined by FPIA and HPLC

环孢素(Cyclosporine A,CSA)是器官移植中有效的免疫抑制剂,然而CsA的肝、肾毒性限制了它的安全应用,而且诊断上与排斥反应不易区别。本文用FPIA和和HPLC法同时测定6例肾移植病人口服CsA后的50份血样中CsA浓度,两法相关系数r=0.85,斜率0.38。研究两法测定CsA浓度比值与肾功能的关系,以及比值对诊断肾毒性和排异反应的临床意义。

FPIA and HPLC methods were simultaneously used to the blood concentrationof cyclosporine of 6 patients of renal transplantation given orally was determined.The correlation of the two methods was analysed (correlation coefficient 0.85, slope0.38). It was found that the ratio of the cyclosporine concentrations determined bytwo methods(FPIA/HPLC)had clinical significance in the discrimination of nephro-toxicity and rejection. For 4 patients with normal renal function. the ratio was1.65-4.87. For 2 patients in acute rejection phase, the ratio was 1.30-4. 19.Nephro-toxicity occured when the ratio was 8.3-14.3.