铬胁迫对黄豆生理特性的FTIR研究和铬积累特征

Physiological Response of Glycine max L. to Cr Stress by FTIR Spectroscopy and Cr Accumulation Characteristics

为了对Cr^(6+)污染土壤修复提供参考依据。通过水培,研究了不同Cr^(6+)水平对黄豆幼苗根、茎、叶的傅立叶红外光谱(FTIR)图谱变化,同时测定Cr^(6+)的亚细胞分布和Cr^(6+)富集的影响。结果表明:幼苗对Cr^(6+)的积累能力与处理浓度呈正比。其叶片富集的Cr^(6+)在细胞壁分布最多(37.14%~63.52%),线粒体和叶绿体最少(<12%)。根组织在1064、1404、1635、2924、3417 cm-1等处峰高呈先升后降的变化趋势,可能是在低Cr^(6+)浓度,通过增加糖类和氨基酸等有机物含量来响应Cr^(6+)胁迫;茎组织在1072、1427、1643、2924、3387 cm-1等处峰高呈现不显著变化趋势;叶组织在1110、1396、1651、2924、3433 cm-1等处峰高先降后升,表明叶中一些富含O-H小分子有机物质(碳水化合物)被运输到根部螯合Cr^(6+)来响应低Cr^(6+)胁迫。研究结果表明,黄豆幼苗能有效地吸收土壤中的Cr^(6+),在Cr^(6+)污染土壤植物修复技术领域,拥有潜在的利用价值。

To provide references for Cr contaminated soil remediation, the authors studied the effect of differentCr6 ＋concentrations on FTIR spectrogram and determined the subcellular distribution of Cr6 ＋and itsaccumulation. The results showed that the accumulation capacity of Cr6 ＋in plant was positively correlated toCr6＋concentration. Cr6＋accumulated in leaves was mainly distributed in cell wall（37.14%-63.52%）, and wasleast distributed in the mitochondria and chloroplasts（12%）. Cr6＋in root first increased then decreased near1064, 1404, 1635, 2924 and 3417 cm-1, which might be caused by the increase of organic contents（e.g.carbohydrates and amino acids） responding to the Cr6＋stress. Cr6＋in stems had no obvious change near 1072,1427, 1643, 2924, 3387 cm-1.Cr6 ＋in leaves near 1110, 1396, 1651, 2924, 3433 cm-1 first decreased thenincreased, indicating that small organic substances containing O-H（carbohydrate） in cotyledons weretransported to the roots firstly and then reacted with Cr6 ＋chelates to reduce the toxicity of Cr6 ＋. These resultsdemonstrate that G. max L. is suitable for phytoremediation of Cr6＋contaminated soils and has potential value inimprovement of soil quality from the aspect of food safety.