紫苏叶提取物对3T3-L1脂肪细胞的影响

Effect of Perillae Folium Extract on 3T3-L1 Adipocytes

目的探讨紫苏叶提取物(Perillae folium extract,PFE)对3T3-L1脂肪细胞的影响。方法将3T3-L1脂肪细胞培养在不同浓度的PFE(100,250和500μg/ml)中,通过MTS法测定PFE的无毒性细胞浓度,通过Western blot法测定过氧化物酶体增殖物激活受体-γ(PPAR-γ)和CAAT/增强子结合蛋白α(C/EBPα)的蛋白表达水平变化,通过RT-PCR法测定PPAR-γ、C/EBPα、固醇调节元件结合蛋白1C(SREBP-1c),硬脂酰辅酶脱氢酶1(SCD1),脂肪酸合成酶(FAS),甘油-3-磷酸酰基转移酶(GPAT)和肉碱棕榈酰转移酶1(CPT1)等基因的mRNA表达水平变化。结果通过MTS法得出PFE的无毒性细胞浓度。在Western blot实验中,PPAR-γ和C/EBPα蛋白量的表达随着PFE浓度的升高而减少。在RT-PCR结果中,PPAR-γ、C/EBPα、SREBP-1c和GPAT等基因的表达随着PFE浓度的升高而减少,SCD1和FAS的基因表达水平也降低,CPT1基因的表达水平是随着PFE的浓度而逐渐增加的。此结果说明PPAR-γ和C/EBPα的基因和蛋白表达是受到抑制的。结论 PFE是通过抑制PPARγ、C/EBPα和SREBP1及其靶基因的表达影响脂肪细胞的生成及形成,调节脂肪组织脂质代谢,对以后研究肥胖症具有一定的参考意义。

Objective To study the effect of Perillae Folium Extract（PFE） on 3T3-L1 adipocytes.Methods 3T3-L1 fat cells were cultured in different concentrations of PFE（100,250 and 500 ug/ml）.The non-toxic concentration of PFE on the cell was measured by MTS.Changes in the expression levels of the PPAR-γ and C/EBPα protein were determined by the Western blot.Changes in the expression levels of mRNA of the PPAR-γ,C/EBPα,SREBP-1c,SCD1,FAS,GPAT and CPT1 gene was measured by RT-PCR.Results The non-toxic cell concentration of PFE was obtained by MTS.In Western blot experiments,the expression of PPAR-γ and C/EBPα protein decreased with increasing PFE concentration.In RT-PCR results,the expression of PPAR-γ,C/EBPα,SREBP-1 c and GPAT gene decreased with the increasing of PFE concentration,and the expression level of SCD1 and FAS gene also decreased,however,the expression of CPT1 gene increased.These results indicated that the expression of PPAR-γ and C/EBPα gene and protein was inhibited.Conclusion PFE affects the production and formation of adipocytes and regulates lipid metabolism in adipose tissue by inhibiting the expression of PPAR-γ,C/EBPα,SREBP1 and their target genes,which has a certain reference value for future study of obesity.