天佛参对奥沙利铂神经毒性大鼠模型的影响

Effects of Tianfoshen Oral Liquid on Oxaliplatin Induced Neurotoxicity in Rats

目的观察天佛参口服液对奥沙利铂神经毒性模型大鼠的影响,并探索其可能机制,进而为其临床应用于防治奥沙利铂神经毒性提供理论基础。方法 Wistar大鼠采用体重分层法将大鼠随机分成空白组、造模组、文拉法辛组和天佛参组。除空白组予5%葡萄糖注射液4mg/kg腹腔注射外,其他各组均采用奥沙利铂4mg/kg腹腔注射,每周2次,连续4周,建立奥沙利铂神经毒性模型。此外,造模组予生理盐水2ml/d灌胃,文拉法辛组予文拉法辛溶液16mg/(kg·d)灌胃,天佛参组予天佛参口服液2ml/d灌胃。每周观察各组大鼠的体重和机械刺激缩足反射阈值(mechanical withdrawal threshold,MWT),最后检测L_4~L_5背根神经节μ、κ阿片受体的表达情况。结果天佛参能增加奥沙利铂神经毒性模型大鼠的体重,提高MWT值,诱导μ、κ阿片受体表达升高。结论天佛参能有效地压制奥沙利铂诱发的末梢疼痛觉过敏症状,其作用机制可能与诱导μ、κ阿片受体表达升高有关。

Objective To observe the effect of Tianfosshen oral liquid on oxaliplatin induced neurotoxicity in rats,and to explore its possible mechanism,further more,to provide a theoretical basis for its clinical application.Methods The weight stratified method was used for dividing 40 wistar rats,randomly and averagely,into blank group,model group,venlafaxine group and Tianfoshen group.Oxaliplatin was administered at 4 mg/kg,twice-weekly for four weeks by intraperitoneal injection to induce peripheral neuropathy in rats,beyond the blank group was administered at 4 mg/kg 5% glucose injection instead.In addition,the model group was administered at normal saline,and the venlafaxine group was administered at venlafaxine 16 mg/（kg·d）,meanwhile the Tianfoshen group was administered at Tianfoshen oral liquid 2 ml/d.We observed weight and mechanical withdrawal threshold（MWT） every week.At the 29 th,the μ and κ opioid receptor expression were analyzed which in L4 ~ L5 dorsal root ganglion.Results Tianfoshen significantly increased the weight and MWT in the rats with oxaliplatin induced neuropathic.Both the μ andκ opioid receptor expression were increscent in the Tianfoshen group compare with the model group.Conclusion Tianfoshen antagonizes oxaliplatin induced neuropathic pain through up-regulation of μ and κ opioid receptor possibly.