标本配穴电针治疗对慢性心肌缺血模型大鼠心肌梗死面积与细胞凋亡指数的影响

Effects of Electro acupuncture of Combined Biao and Ben Acupoints treatment on Myocardial Infarction Area and Apoptosis Index in Rats with Chronic Myocardial Ischemia

目的观察标本配穴针刺治疗对慢性心肌缺血大鼠心肌梗死面积及心肌细胞凋亡的影响,探讨其保护作用及机制。方法将70只SPF级Wistar雄性大鼠根据随机数字表法分为7组,每组10只。采用腹部皮下注射ISO制作心肌缺血模型。LY294002组和IGF-1组分别在造模当日给予LY294002溶液和IGF-1溶液注射,所有针刺组在造模前进行电针针刺预处理。试验结束后采用Evans Blue-TTC双染法检测心肌梗死面积指标IS/LV(梗死心肌面积/左心室心肌)和ARR/LV(危险心肌/左心室心肌),TUNEL技术测定心肌细胞AI(凋亡指数)。结果其他各组IS/LV、ARR/LV和AI均比正常组相明显增高,差异有显著性意义(P<0.05或P<0.01)。IGF-1组、内关组和标本配穴组IS/LV、ARR/LV和AI均比模型组有所下降,差异有显著性意义(P<0.01);LY294002组和标本配穴+LY294002组的IS/LV、ARR/LV和AI明显升高,差异有统计学意义(P<0.01或<0.05)。IGF组IS/LV、ARR/LV和AI比标本配穴组有所降低,但差异无统计学意义(P>0.05);内关组、LY294002组和标本配穴+LY294002组的IS/LV、ARR/LV和AI高于标本配穴组,差异有显著性意义(P<0.01)。结论标本配穴电针治疗在对大鼠慢性心肌缺血梗死面积及细胞凋亡的改善作用上优于单取内关穴,其作用机制有可能是通过激活PI3K/Akt通路起到保护心肌的作用。

Objective To observe the effect of Electro acupuncture of combined Biao and Ben acupoints treatment on the myocardial infarct size and cardiomyocyte apoptosis in rats with chronic myocardial ischemia,and to explore its protective effect and mechanism.Methods According to the random number table method,70 normal Wistar male rats were divided into 7 groups.Adopt isoproterenol hydrochloride（ISO） injected to make the chronic myocardial ischemia rat model,the LY294002 group and IGF-1 group were respectively given LY294002 solution and IGF-1 solution inject in day of first moldeling,all acupuncture groups adopt Electroacupuncture pretreatment before modeling.After the intervention,use the Evans Blue-TTC double staining method to detect myocardial infarct size index： IS/LV（Infarct Size,IS; Left Ventricle,LV） and ARR/LV（Area At Risk,AAR）,use the TUNEL technology to detect the cardiomyocyte apoptosis index（AI）.Results Compared with the normal group,the other groups＇ IS/LV,ARR/LV and AI were significantly increased,the difference was significant（P〈0.05 or P〈0.01）; Compared with model group,the IGF-1 group the Neiguan group and the electro acupuncture of combined Biao and Ben acupoints Treatment groups＇ IS/LV,ARR/LV and AI had declined,the difference was significant（P〈0.01）; the LY294002 group and the electro acupuncture of combined Biao and Ben acupoints Treatment ＋ LY294002 groups＇ IS/LV,ARR/LV and AI were significantly increased,the difference had statistically significant（P〈0.01 or 0.05）.Compared with the electro acupuncture of combined Biao and Ben Acupoints Treatment groups,the IGF group＇s IS/LV,ARR/LV and AI was decreased,but the difference had not statistically significant（P〈0.05）; The Neiguan group,the LY294002 group and the electro acupuncture of combined Biao and Ben acupoints treatment ＋ LY294002 groups＇ IS/LV,ARR/LV ratio and AI was higher than the Electro Acupuncture of Combined Biao and Ben Acupoints Treatment group＇s,the difference was significant（P〈0.01）.Conclusion Compared with the single take Neiguan acupoint,the electro acupuncture of combined Biao and Ben acupoints treatment of the acupoints has more advantages in improving the infarct size and apoptosis of chronic myocardial ischemia in rats.Its mechanism may be through the activation of PI3 K/Akt pathway to protect the role of myocardium.