草莓根腐病的病原菌分离鉴定及拮抗菌CM3的抑制作用研究

Isolation and Identification of Pathogenic Fungi of Strawberry Root Rot and the Inhibition of Antagonistic Bacteria CM3 on These Fungi

从草莓根腐病病株根部成功分离了2株真菌FC2016-3和FC2016-7,经过鉴定确定其为根腐病的病原菌——尖孢镰刀菌(Fusarium oxysporum)。解淀粉芽孢杆菌(Bacillus amyloliquefacien)CM3对菌株FC2016-3和FC2016-7有明显的抑制作用,也对其他土传病害病原菌有较好的抑制效果。盆栽实验结果显示,菌株CM3对尖孢镰刀菌FC2016-7引起的草莓根腐病的防治效果达到了64.86%,而且还具有促进草莓根部生长的能力。研究表明菌株CM3是一株既能防病也能促生的菌株,适合开发成微生物制剂。

In this paper,two fungi FC2016-3 and FC2016-7 were successfully isolated from the diseased root of strawberry,andidentified as Fusarium oxysporum. Bacillus amyloliquefacien CM3 presented obvious inhibitory effect on FC2016-3 and FC2016-7,and alsohad a promising inhibitory effect on other soil borne pathogens. The results of pot experiment showed that the control effect of strain CM3 onstrawberry root rot caused by F. oxysporum FC2016-7 was up to 64.86%,and strain CM3 also demonstrated the ability of promoting rootgrowth of strawberry. This study reveals that strain CM3 is a strain that can prevent soil borne disease and simultaneously promote plant growth,therefore,it is appropriate for the development of microbial preparation.