牛布鲁氏菌病不同检测方法比较与试剂质控

Comparison of Different Methods for Detection of Bovine Brucellosis and Reagent Quality Control

选用国标等同阳性血清,对虎红抗原进行质控,经三次重复试验,最终选定稀释度为1/45时反应阳性,1/55时反应阴性。ELISA试剂盒质控选择国标等同阳性血清,稀释度为1/16时反应阳性,1/64时反应阴性。利用虎红平板凝集试验(RBT)、试管凝集试验(SAT)和抗体竞争ELISA试验三种方法,对甘肃省河西地区4个奶牛场共计1167份奶牛血清进行了检测,结果表明:三种试验方法的符合率分别为:抗体竞争ELISA试验与虎红平板凝集试验符合率为52.7%,与试管凝集试验符合率为34%。虎红平板凝集试验与试管凝集试验符合率为72.1%。抗体竞争ELISA试验方法最为敏感。

In this paper, the positive serum equivalent to the national standard was selected and the quality control of the Rose Bengal test antigens was tested. Three repeated experiments later, the reaction was positive when dilution degree was at 1/45, and the reaction was negative at 1/55. The same national standard serum was selected for ELISA kit quality control, and the dilution was positive at 1/16 and negative at 1/64. A total of 1167 dairy cows serum from 4 dairy farms in Hexi area of Gansu Province were tested by three methods named Rose Bengal test （RBT）, Serum agglutination test （SAT） and antibody competitive ELISA test. The results show that the accuracy of three test methods are： antibody competitive ELISA test with Rose Bengal test（RBT） coincidence rate was 52. 7%, and Serum agglutination test （SAT） coincidence rate was 34%. The combination of the Rose Bengal test （RBT） and Serum agglutination test （SAT） was 72.1%. Antibody competition ELISA test method is most sensitive.