直接测汞仪法测定尿中汞国标方法的研制

National standard method of determining mercury in urine by a direct mercury analyzer

目的建立一种快速简便、能满足生物监测国家标准要求的尿中汞的测定方法。方法采用直接测汞仪对尿中汞的测定情况进行考察和试验。结果尿中汞含量在1.6~100μg/L时测得的峰面积和汞浓度之间呈良好的线性关系,相关系数>0.999。方法检出限为0.5μg/L,方法定量下限为1.6μg/L。批间、批内测定的相对标准偏差均小于5%。对10、30、60μg/L三个浓度水平的尿样,加入与原浓度相近的汞浓度的加标回收率为91.6%~99.9%,对有证参考物质的测定及与冷原子吸收法的比对结果满意。结论直接测汞仪法作为一种简单、快速的测定方法能满足生物材料中化学物质测定方法的要求,可以用于职业接触者尿中汞的测定。

Objective To establish a rapid and simple method for the determination of mercury in urine, which can meet the requirements of national standard of biological monitoring. Method A direct mercury analyzer was used to investigate and test the determination of mercury in urine. Results There was a good linear relationship between the peak area and mercury concen- tration when the mercury concentration in urine ranged between 1.6 μg/L and 100μg/L, and the correlation coefficient was above 0.999. The detection limit and quantitative limit of the method were 0.5 μg/L and 1.6 μg/L respectively. The inter-batch and intra -batch relative standard deviations （ RSDs ） were both less than 5%. Spiking three concentration levels of mercury （ including 10μg/L, 30μg/L and 60μg/L） in urine samples, the recoveries of standard addition were 91.6%-99.9%. The results were con- sistent with reference material certificates and cold atomic absorption method result. Conclusions This direct mercury analyzer method, as a simple and rapid method for the determination of mercury, can meet the requirements of detection method of chemical substance in biomaterial ; and hence, it can be used for the determination of mercury in urine of occupational exposure.