UHRF1在宫颈癌组织中的表达及其临床意义

Expression of UHRF1 in cervical cancer and its clinical significance

目的探讨宫颈癌组织中的UHRF1表达及其临床意义。方法收集本院2013年1月至2016年6月经手术切除的宫颈癌组织89例、正常宫颈组织68例及宫颈上皮内瘤样病变(CIN)组织109例(CINⅠ29例、CINⅡ33例和CINⅢ47例),采用实时定量PCR(QPCR)法检测以上组织中UHRF1的表达情况,分析其表达与宫颈癌临床病理参数的关系(年龄、淋巴结转移、FIGO分期、肿瘤大小、组织学分级和病理类型),采用受试者工作曲线(ROC)分析UHRF1诊断不同宫颈病变的效能。结果宫颈癌组织中的UHRF1水平为3.086±2.327,均高于正常宫颈组织和CIN组织,差异有统计学意义(P<0.05)。UHRF1表达与年龄、淋巴结转移及病理类型无关(P>0.05),与FIGO分期和组织学分级有关(P<0.05),其中Ⅱ、Ⅲ期的UHRF1表达水平为3.793±2.410,高于Ⅰ期的1.764±1.443,而G_2、G_3级的UHRF1表达水平为3.811±2.330,高于G_1级的1.230±0.784,以上差异有统计学意义(P<0.05)。ROC曲线分析显示UHRF1区分宫颈癌与正常宫颈、区分宫颈癌与CIN及从所有受试者中区分出宫颈癌的AUC分别为0.803(灵敏度69.66%、特异度85.29%),0.734(灵敏度66.29%、特异度73.39%)和0.760(灵敏度66.29%、特异度79.10%)。结论宫颈癌组织中UHRF1为高表达,与组织学分级和FIGO分期有关,且诊断宫颈癌的效能较好,可能成为宫颈癌诊断和病情预测的新型分子标记物。

Objective To investigate the expression of UHRF1 in cervical cancer and its clinical significance. Methods From January 2013 to June 2016, 89 cases of cervical cancer, 68 cases of normal cervical tissue and 109 cases of cervical intraepitheli- al neoplasia （CIN）, including 29 cases of CIN I , 33 cases of CIN II and 47 cases of CIN III, were collected. The expression of UHRF1 in the above tissues was detected by real-time quantitative PCR （QPCR）. The relationship between the expression of UHRF1 and the clinicopathological parameters of cervical cancer （ age, lymph node metastasis, FIGO staging, tumor size, histological grade and pathological type） was analyzed. The receiver operating characteristic curve （ROC） was used to analyze the value of UHRF1 in the diagnosis of different cervical lesions. Results The level of UHRF1 in cervical cancer tissues was 3.086±2. 327, which was higher than that of normal cervical and CIN tissues, and the difference was statistically significant （P〈0. 05）. The expression of UHRF1 was not related to age, lymph node metastasis and pathological type （P〉0. 05）, but associated with FIGO stage and histological grading （P 〈0. 05）. The expression level of UHRF1 in stage II -III was 3. 793±2. 410, higher than 1. 764±1. 443 in stage I , and the expression level of UHRF1 in G2-G3 stage was 3. 811±2. 330, higher than that 1. 230±0. 784 in G1 stage （P〈0. 05）. ROC curve analysis showed that AUC of UHRF1 to distinguish cervical cancer from normal cervix, CIN and all subjects were 0. 803 （ sensitivity 69. 66%, specific- ity 85. 29% ）, 0. 734 （ sensitivity 66. 29%, specificity 73.39% ） and 0. 760 （ sensitivity 66. 29%, specificity 79. 10% ）. Conelusion UHRF1 is highly expressed in cervical cancer tissue, and is related to histological grade and FIGO stage. It is effective in diagnosing cervical cancer. It may become a new molecular marker for diagnosis and prediction of cervical cancer.