金耳子实体多糖硫酸化修饰研究

Sulfation of Polysaccharides from Fruiting Bodies of Tremella aurantia

采用氯磺酸法对金耳(Tremella aurantia)子实体多糖进行硫酸化修饰,通过单因素实验考察了溶剂、氯磺酸用量、反应温度及时间对多糖硫酸化的影响并研究了不同取代度的硫酸化金耳多糖体外抗氧化活性和抑制L1210肿瘤细胞增殖的作用。以取代度为指标,金耳多糖硫酸化的最佳条件是反应溶剂为N,N-二甲基甲酰胺,多糖残基与氯磺酸的摩尔比为1∶4,反应温度为60℃,反应时间为3h,在此条件下,产物取代度为1.48。此外,随着多糖取代度的增加,硫酸化金耳多糖对过氧化氢、羟自由基的清除率显著提高,对L1210细胞的抑制率也明显升高,说明硫酸化多糖的抗氧化活性和体外抑制肿瘤细胞的活性与其硫酸化的取代度相关。

Sulfation modification is known to improve biological activities of polysaccharides, and the potency of biological activities is found to be closely related to the degree of substitution （DS） for sulfated derivatives. Using the chlorosulfonic acid method, polysaccharides from fruiting bodies of T. aurantia were sulfated by different quantities of chlorosulfonic acid under different solvent conditions, temperatures and incubation time. Then the sulfated polysaccharides produced under different conditions were determined for their DS, hydrogen peroxide and hydroxyl radical scavenging activities, and proliferation inhibition effect on cancer cell line L1210 in vitro, respectively. The results showed that as the DS increased, the tested biological activities of sulfated polysaccharides also increased, suggesting a positive correlation between DS and the tested biological activities of sulfated polysaccharides. Using DS as the criterion, the optimized sulfation condition for T. aurantia fruiting body polysaccharides was as follow., using DMF as the solvent, the molar ratio of polysaccharide residue and chlorosulfonic acid as 1 ： 4, reaction temperature at 60 ℃ and reaction time for 2.5 h. Under the optimized sulfation condition, the DS of the product reached 1.48.