不同草莓品种酚类物质含量及抗氧化能力研究

Content of Phenolic Compounds and Antioxidant Capacity of Different Strawberry Varieties

为研究山西晋中地区草莓不同品种酚类物质含量及其抗氧化能力,并为草莓栽培、育种、加工利用提供相关依据,采用Folin-Ciocalteu法测定总酚含量、Folin-Denis法测定单宁酚含量、正丁醇-盐酸法测定原花色素含量、香草醛-硫酸比色法测定花青素含量,采用羟基自由基清除力、DPPH自由基清除力和FRAP铁还原值3种方法测定抗氧化能力。结果表明:‘枥乙女’的总酚、单宁酚、花青素、原花色素含量均为最高。综合3个抗氧化性指标,‘枥乙女’的抗氧化能力较强。主成分分析表明,前3个主成分基本概括了酚类物质含量和抗氧化能力的主要信息,累积贡献率为92.59%。16个草莓品种中,‘枥乙女’综合评分最高,是促进草莓消费市场和产业发展重要品种资源。

To study the content of phenolic compounds and antioxidant capacity of different strawberry varieties in Jinzhong Prefecture of Shanxi Province while provide a basis for the reasonable development and effective utilization of plant polyphenols in strawberry, total phenols content was determined by Folin- Ciocaheu method, tannin phenol content was determined by Folin-Denis method, proanthocyanidin content was determined by inn-butanol-HCl method, anthoeyania content was determined by vanillin-sulfuric colorimetry, antioxidant activity was determined by hydroxyl radical scavenging method, DPPH radical scavenging method and FRAP iron reducing method, and then the data were mined by variation analysis, correlation analysis and principal component analysis. It was indicated that the content of total phenol, tannin, anthocyanin, and proanthocyanidin in ＇Tochiotome＇ was the highest while that of ＇05-1-5＇ was the lowest, antioxidant capacity of ＇Tochiotome＇ was the highest while that of ＇05-1-5＇ was the lowest through comprehensive analysis of 3 antioxidant indexes, phenolics content and antioxidant capacity of strawberry were significantly correlated, and by principal component analysis it was showed that the first three principal components basically outlined the main information of phenolics content and antioxidant capacity with a cumulative contribution rate of 92.59%, and ＇ Tochiotome＇ had the best comprehensive quality. In conclusion, ＇Tochiotome＇ is an important genetic resource to cultivate new strawberry varieties with high biological activity and to promote the development of strawberry industry.