固相微萃取-气相色谱-质谱联用法检测物流过程中液态乳质量

Detection of quality of liquid milk during the progress of logistics by solid-phase microextraction-gas chromatography-mass spectrometry

目的建立固相微萃取-气相色谱-质谱联用法检测物流运输前后液态乳质量。方法利用顶空固相微萃取,结合气质联用技术和吹扫捕集法分析液态乳制品中挥发性成分,检测牛奶中的氯仿和脂质降解程度。结果液态乳制品样品中有7种挥发性成分具有指示作用,主要是醛和酮;使用顶空分析能够量化氯仿污染物,脂肪分解的程度可以通过测量游离脂肪酸来量化。结论脂肪酸酶解产物可作为液态乳制品物流过程质量控制的生物标志物,固相微萃取的灵敏度和重现性满足液态乳制品物流过程的质量控制的要求。

Objective To detect the quality of raw milk before and after the logistics by solid-phase microextraction-gas chromatography-mass spectrometry （SPME-GC-MS）. Methods The volatile components in liquid dairy products were analyzed by headspace solid-phase microextraction, combined with GC-MS and purge and trap method to detect chloroform and lipid degradation in milk. Results There were seven kinds of volatile components in the liquid dairy product samples, which were mainly aldehydes and ketones. Through headspace analysis to quantify chloroform contaminants, the extent of lipolysis could be quantified by measuring free fatty acids. Conclusion Fatty acid hydrolyzate can be used as a biomarker for the quality control of liquid dairy product during the logistics process. The sensitivity and reproducibility of solid-phase microextraction can meet the quality control requirements of liquid dairy product during the progress of logistics.