基于网络药理学研究当归芍药散防治阿尔茨海默病的作用机制

Study on Danggui-Shaoyao-San for treatment of Alzheimer's disease based on network pharmacology

目的探究当归芍药散防治阿尔茨海默病的作用机制。方法选取当归芍药散中的当归、白芍、白术、川芎、茯苓、泽泻为研究对象,应用TCMSP数据库对6味中药主要化学成分进行筛选;使用TCMSP数据库,对筛选出的化合物及阿尔茨海默病进行靶点预测,选取当归芍药散与阿茨海默病一致的6个靶点蛋白作为后续研究对象,通过生物分子功能注释系统(MAS 3.0)及KEGG通路数据库进行通路注解及分析,得到当归芍药散治疗阿茨海默病的相关靶点通路预测图及相关信号通路。结果从当归芍药散中筛选出35个化合物,可作用于阿尔茨海默病6个潜在的蛋白靶点,找出靶点的相关通路22条。作用通路涉及阿尔茨海默病发病机制相关的钙信号途径、炎症、免疫调节、细胞与细胞之间的信号交流、肌动蛋白细胞骨架的调节等各个环节,6味中药既有共同的成分及作用靶点、通路,又各有偏重,各通路间通过共有靶点连接,显示出不同成分间的多靶点、多途径的协同作用。结论预测出当归芍药散治疗阿尔茨海默病可能与其调节炎症、免疫系统、钙信号、细胞与细胞之间的信号交流等机制有关。

Objective To explore the mechanism of Danggui-Shaoyao-San（DSS）for the treatment of Alzheimer＇s disease.Methods Angelicae Sinensis Radix,Paeoniae Radix Alba,Chuanxiong Rhizoma,Atractylodes Macrocephala Koidz,Poria Cocos,and Alisma Orientale were selected as the research object.TCMSP database was applied to screening the main chemical components of DSS.Forecast the potential targets using TCMSP database for Alzheimer＇s disease and the main chemical components,and selected six consistent target proteins as a follow-up object.Through the MAS 3 and KEGG pathway database,path annotation and analysis were carried out.Finally,DSS related target pathway for the treatment of Alzheimer＇s disease prediction map and the related signaling pathways were obtained.Results Six potential protein targets and 22 targets related pathways related with Alzheimer＇s disease were identified in DSS 35 components,including calcium signaling pathways,inflammation,immune system,signal exchange between cells and cells,the regulation of actin cytoskeleton,and so on.The targets and signaling pathways among the six flavour Chinese drug components,and each have lay particular stress on,through mutual targets for connections between various pathways,sho ws a different composition between the target and way of synergy.Conclusion Danggui-Shaoyao-San for treatment of Alzheimer＇s Disease may be linked to regulate inflammation,immune system,calcium signal and signal exchange between cells and cell life activities.