大豆UV-B光受体GmUVR8基因的生物信息学分析

Bioinformatics Analysis on UV-B Photoreceptor Gene of GmUVR8 in Soybean

UV-B影响大豆的生长发育,降低大豆的产量,但能够促进大豆类黄酮化合物合成,提高植物对病虫害的抵抗力。本研究利用生物信息学方法系统分析了大豆UV-B光受体UVR8基因及其编码蛋白质的特性。结果显示,大豆中存在4个GmUVR8蛋白质,均为亲水性蛋白质,位于细胞核中,尽管它们在大小上存在差异,但是都包含多个保守的RCC1结构域,形成了完整或不完整的七叶β-折叠的结构,与拟南芥的折叠方式极为相似。大豆基因组中这4个GmUVR8编码基因长度不一,分别位于4条染色体上,含有相同的外显子数,具有相同的编码方式。同源序列比对显示GmUVR8c与拟南芥最为相似,而聚类分析显示GmUVR8具有一定特异性。本研究为后期系统分析大豆UV-B光形态建成分子机制和GmUVR8基因功能提供了理论依据。

UV-B affected the growth and development of soybean, and caused a decline of yield. However, UV-B could pro- mote the synthesis of flavonoids in soybean and enhance its resistance to insect pests. In this study, the characteristics of soy- bean UV-B photoreceptor GmUVR8 genes and their encoded proteins were systematically analyzed by bioinformatics methods. The results showed that there were four GmUVR8 proteins with different sizes in soybean. GmUVR8 which located in nucleus were hydrophilic proteins. All GmUVR8 proteins had several RCC1 domains, formed a complete or incomplete seven-bladed β-propeller which similar with AtUVR8 protein. Four GmUVR8 genes with different lengths and genome locations had the same exons numbers and coding modes. Homologous sequence alignment showed that GmUVR8c was most homologues with AtU- VR8. But the cluster analysis showed GmUVR8 had its specificity. This study provides a theoretical basis for analysis of the molecular mechanisms of soybean UV-B morphogenesis and GmUVR8 genes functions in future.