广灵驴成纤维细胞系的建立及其相关生物学特性研究

Establishment and Biological Characteristics Analysis of Fibroblast Cell Line of Guangling Donkey

试验旨在建立广灵驴成纤维细胞库,以期在细胞水平上对广灵驴进行保护。本研究以1月龄广灵驴耳缘皮肤组织为试验材料,采用组织块贴壁法进行成纤维细胞培养,建立了广灵驴耳缘皮肤组织成纤维细胞系,并对其相关生物学特性进行了鉴定。结果发现,试验所建立的广灵驴成纤维细胞系大多数细胞呈长梭形,部分细胞呈三角状或星型。在培养过程中,广灵驴原代成纤维细胞在贴壁4d时开始有细胞从组织边缘游离出来,贴壁14d后,细胞汇合率达到80%,可以进行第一次传代培养;细胞生长态势良好,生长曲线呈典型的S型曲线;细胞冻存复苏后活率有所下降,但生长状态良好。细胞分裂中期染色体数2n=62,说明成功建立了广灵驴成纤维细胞系。通过本方法建立的广灵驴成纤维细胞系为后续广灵驴的相关研究奠定了基础。

This study was aimed to establish the fibroblast cell line of Guangling donkey,and protect Guangling donkey at the cell level.In the experiment,the fibroblast cell culture was performed on auricular skin of Guangling donkey using the method of tissue-sticking.The fibroblast cell line was established,and its biological characteristics were studied.The results showed that the shape of most fibroblasts in Guangling donkey were spindle,however,some of them were triangle or star.During the process of culturing,the primary fibroblasts began to dissociate from the edge of the tissue at 4 days of adherence.After 14 days of adherence,the cell confluence rate reached about 80%,and the cells could be subcultured for the first time.The measured cell growth curve which was the typical S-shaped curve showed that the cell growth was in good con-dition.After cryopreservation,the survival rate decreased.The quantity of chromosomes 2 n=62 indicated that the fibroblast cell line of Guangling donkey was successfully established.The fibroblast cell line established by this kind of method provided the foundation for the related research of Guangling donkey.