天津地区2型猪链球菌的分离鉴定及耐药性分析

Isolation, Identification and Drug Resistance Analysis of Streptococcus suis Serotype 2 in Tianjin

为了解天津地区2型猪链球菌的流行及耐药情况,本研究收集该地区部分规模化养猪场疑似猪链球菌病病料317份,通过病原分离培养、染色特性观察、生化试验、PCR扩增等方法进行鉴定,并对分离菌进行致病性及药敏试验。结果从样品中分离鉴定出11株2型猪链球菌。对小鼠的致病力试验结果显示,应用0.5×10~8 CFU/mL的细菌攻击小鼠,11株2型猪链球菌分离株中有6株具有致死性,其中1株致死率较强,5株致死率较弱。药敏试验结果表明,11株分离菌对9种临床常见药物均表现出很强的耐药性,其中对阿米卡星、四环素和强力霉素耐药性最强,耐药率达到100.00%;且所有分离株均呈多重耐药,其中4个分离株为9重耐药,占36.36%(4/11)。本试验结果表明,天津地区存在2型猪链球菌感染情况,且对多种抗菌药均产生了耐药性,应引起养殖户的高度重视,在防制猪链球菌病时,应有针对性地合理、科学、规范用药和交替用药。

In order to investigate the prevalence and drug resistance of Streptococcus suis serotype2（SS2）in Tianjin,317 samples of suspected Streptococcus suis disease were collected from largescale pig farms in this area.11 SS2 strains from the samples were identified by cultural character,dyeing property analysis,biochemical test and PCR amplification.The pathogenicity and drug susceptibility test of the isolates were also carried out.The pathogenicity test result showed that 6 strains were lethal to mice in the 11 SS2 isolate strains,including 1 strain with high lethality and 5 strains with low lethality.Drug sensitivity test result indicated the 11 isolates were highly resistant to clinical drugs,the resistance rate was up to 100.00%to amikacin,tetracycline and doxycycline.All of the 11 isolates were multidrug resistant,and 4 SS2 isolates were resistant to 9 drugs among them,which was accounting for 36.36%（4/11）.The results showed that SS2 occurred in Tianjin area and the pathogenic bacteria were resistant to many antibacterials.Farmers shouldpaid close attention to this,rational,scientific,standardized medication and alternative medication when prevented the Streptococcus suis disease.