摘要
芍药苷具有抑制炎症和镇痛的作用,在治疗炎症疼痛方面具有重要价值,但其作用机制尚不明确。本研究发现,弗氏完全佐剂诱导小鼠炎症疼痛模型,用80 mg/kg芍药苷腹腔注射能有效缓解疼痛。检测发现芍药苷治疗后,小鼠机械性痛阈与热板痛阈均明显升高(机械性痛阈值:由6.38±1.00 g提高至8.31±0.81 g;热板痛阈值:由5.78±0.76 s提高至9.90±1.58 s);同时抑制外周炎症因子TNF-α等的释放(由708.71±46.55 pg/m L降低至588.65±16.02 pg/m L);免疫组织化学检测发现,芍药苷能有效抑制脊髓背角小胶质细胞的激活;NO检测结果发现,脊髓部位NO合成降低(3.55±0.28μmol/L·g-1Pro降至2.25±0.71μmol/L·g-1Pro);Western印迹检测证实,脊髓部位i NOS在使用芍药苷后,表达恢复正常水平。同时发现,Akt-NF-κB信号可能参与芍药苷的镇痛作用。上述结果提示,芍药苷缓解慢性炎症疼痛可通过抑制炎症因子释放,也通过抑制脊髓小胶质细胞的激活,而此过程依赖抑制Akt-NF-κB信号的激活。
Anti-inflammatory and paregoric effects of paeoniflorin( PF) had great medical value in analgesia. This study aims to clarify the effect of PF on inflammation-induce pain,and its potential molecular mechanism. ICR mice intraplantar injected with 20 μL Freund's adjuvant incomplete( CFA)were used as inflammatory pain models. PF attenuated mechanical and thermal pain scores( MPWT: Sham12. 43 ± 1. 04 g,CFA 6. 38 ± 1. 00 g,CFA + PF 8. 31 ± 0. 81 g; TPWT: Sham 14. 60 ± 1. 80 s,CFA 5. 78± 0. 76 s,CFA + PF 9. 90 ± 1. 58 s),and inhibited the production of TNF-α( TNF-α: Sham 523. 84 ±57. 59 pg/m L,CFA 708. 71 ± 46. 55 pg/m L,CFA + PF 588. 65 ± 16. 02 pg/m L). The activation of microglia and NO production was inhibited by PF. Western blotting results showed that i NOS decreased after PF treatment,which was involved in the Akt-NF-κB signaling pathway. These results suggested that PF could efficiently attenuate inflammatory pain in vivo,and inhibited the increase of pro-inflammatory factors,the activation of microglia in the spinal cord,which was dependent on inhibiting the Akt-NF-κB signaling pathway.
作者
洪嘉琪
史家欣
敖欢
章鉴东
翁依佳
朱佳
HONG Jia-Qi, SHI Jia-Xin, AO Huan, ZHANG Jian-Dong, WENG Yi-Jia, ZHU Jia(Department of Microbiology and Immunology, Jiaxing College Sehool of Medicine, Jiaxing 314000, Zhejiang , Chin)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2018年第3期325-333,共9页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家级大学生创新创业训练项目(No.201710354010)
国家自然科学基金项目(No.81501043)资助~~