多指标评分法优选野马追抗动脉粥样硬化有效部位的乙醇提取工艺

Optimization of Eehanol Extraction Technology of Anti-atherosclerosis Effective Fraction in Lindley eupatorium by Multiple Index Scoring Method

目的:优化野马追抗动脉粥样硬化有效部位的乙醇提取工艺。方法:以金丝桃苷、槲皮素、山柰素、棕矢车菊素含量为指标,考察乙醇体积分数、液料比、提取时间、提取次数对野马追药材乙醇提取工艺的影响;采用多指标评分法,设计正交试验法优选提取工艺,并进行验证试验。结果:优化的提取工艺为采用70%乙醇为提取溶剂,按料液比12∶1加入,回流提取3次,每次2 h;3批次验证试验结果显示,所得4种指标成分的含量均较高,平均值分别为5.305 3、1.560 1、1.986 5、6.703 6 mg/g(RSD<1.1%,n=3),结果稳定可靠,且与正交试验结果一致。结论:优化的野马追乙醇回流提取工艺稳定可靠,可操作性好,能源消耗与成本更适合工业化生产,为下一步纯化、富集野马追抗动脉粥样硬化有效成分提供了工艺基础。

OBJECTIVE： To optimize the ethanol extraction process of anti-atherosclerosis effective fraction of Lindley eupatorium. METHODS：Using the contents of hyperin,quercetin,kaempferol and jaceosidin as indexes,the effects of ethanol volume fraction,liquid-solid ratio,extraction time and extraction times on ethanol extraction technology were investigated. By multiple index scoring method,orthogonal test was designed to optimize extraction technology,and validation test was conducted.RESULTS：The optimal extraction technology was 70% ethanol as extraction solvent with solid-liquid ratio of 12∶1,reflux extracting for 3 times,2 h each time. Results of 3 batches of validation tests showed that the contents of 4 marker components were in high level,being 5.305 3,1.560 1,1.986 5,6.703 6 mg/g in average（RSD〈1.1%,n=3）. Results of validation tests were stable and reliable,and were in good agreement with the results of orthogonal tests. CONCLUSIONS：The optimized ethanol reflux extraction technology of L. eupatorium is stable and reliable,and performs well. Energy consumption and cost are suitable for industrial production. The results provide technology basis for further purification and enrichment of anti-atherosclerosis effective components of L. eupatorium.