天麻硫熏标志物p-hydroxybenzyl hydrogen sulfite的定量分析及稳定性研究

Quantitative analysis and stability of p-hydroxybenzyl hydrogen sulfite in sulfur-fumigated Gastrodiae Rhizoma

特征硫熏标志物研究已成为中药质量控制的有效辅助手段。该研究建立了UPLC-MS/MS测定天麻硫熏产物p-hydroxybenzyl hydrogen sulfite的定量分析方法。应用所建立的方法对37批天麻药材进行筛查,发现27批样品是经过硫熏处理的,硫熏较严重的为云南产区的天麻样品。通过考察不同贮藏时间和加热处理下p-hydroxybenzyl hydrogen sulfite成分的稳定性,发现药材中该成分含量在8个月的贮藏期内以及正常加热处理（50,60,70,80℃）条件下是稳定可靠的。以上研究表明该硫熏产物可作为天麻硫熏标志物,具有灵敏度高、选择性好和稳定性好,为天麻质量控制和硫熏药材筛查提供参考。

Studies on the characteristic chemical markers of sulfur fumigation have become an effective auxiliary way for quality control of traditional Chinese medicine. This study established a quantitative analysis method for the determination of p-hydroxybenzyl hydrogen sulfite（ p-HS） in Gastrodiae Rhizoma（ GR） based on UPLC-MS/MS. Then,37 batches of GR were screened,and the results showed that 27 batches of them were sulfur-fumigated. Especially,the GR samples in Yunnan producing areas were sulfur-fumigated more seriously. Based on the stability of p-HS after different storage time and heat treatment methods,it was found that the content of p-HS was stable and reliable in the storage period of 8 months and under normal heat treatment（ 50,60,70,80 ℃） conditions. In conclusion,this study shows a high sensitivity,good selectivity and good stability of p-HS,which can provide reference for the quality control and sulfur fumigation screening of GR