基于PREC-IDA-EPI技术的黄芩中黄酮成分研究

Qualitative characterization of flavonoids in Scutellariae Radix by using PREC-IDA-EPI

黄酮是黄芩的主要活性成分,具有抗肿瘤、抗炎和抗菌等药理作用。黄芩中黄酮成分数量多,通常成分含量较低,常规的质谱全扫描模式较难快速识别低含量的黄酮成分。母离子扫描-信息依赖-增强型子离子扫描（PREC-IDA-EPI）技术靶向提供母离子和碎片离子信息,降低了基质效应对低含量化合物检出的影响可快速准确地完成对化合物的识别。因此该研究在对黄芩研究相关文献进行了调研总结后,明析了黄芩中已发现的黄酮成分和可能存在的黄酮成分。然后基于母离子扫描-信息依赖-增强型子离子扫描（PREC-IDA-EPI）技术建立了针对黄芩中黄酮苷元和其黄酮氧苷的靶向UPLC-MS/MS分析方法,并将其应用于黄芩提取物中的黄酮成分分析。利用该方法共对黄芩中的97个黄酮成分进行了鉴定,包括29个苷元,68个氧苷。黄芩中鉴定的97个化合物为阐明黄芩药效物质基础提供了支持。基于PREC-IDA-EPI技术制定待测化合物的个体化检测方案可快速有效用于未知化合物结构鉴定。

Flavonoids are the most abundant constituents and induce these the rapeutic effects against inflammation,gastrointestinal infections,cardiovascular diseases,and respiratory. Most of these flavonoids have low content in Scutellarie Radix. It was difficult to detect some minor compounds by using LC-MS method with full scan. Based on the review of flavonoids that had been extracted from Scutellariae Radix,a method with PREC-IDA-EPI technique was developed and applied to Scutellariae Radix by using UPLC-MS/MS.A total of 97 flavonoids were identified,including 29 aglycones and 68 O-glycosides. This study laid the foundation for pharmacodynamicss of Scutellariae Radix. It is believed that an individual detection scheme based on the PREC-IDA-EPI technique could be used to identify unknown compounds.