摘要
目的:研究葡萄糖酸锌(ZnG)和氯化锰(MnCl2 )单独及联合对二氧化硅(SiO2 )致巨噬细胞某些生化指标变化的影响。方法:采用肺灌洗法获得肺泡巨噬细胞纯化后(1×10. 9/L)与SiO2 ,同时加入不同浓度的ZnG、MnCl2 以及ZnG +MnCl2 共同培养18h后,分别检测巨噬细胞中的丙二醛(MDA)的含量、超氧化物歧化酶(SOD)的活性以及细胞培养液中乳酸脱氢酶(LDH)的活性。结果:ZnG和Mncl2 ,以及二者联合使用在体外可以降低SiO2 粉尘致巨噬细胞的NDA的含量以及细胞培养液中LDH的活性,提高SOD的活性,二者共同作用效果更明显,ZnG和MnCl2最佳组合剂量是ZnG(1. 5mg/L) +MnCl2 (0 . 2 5mg/L)。结论:ZnG和MnCl2 以及二者联合应用在体外可拮抗SiO2 粉尘所致巨噬细胞的细胞毒性,降低MDA含量,提高SOD活性。
Objective:To investigate the effect of Zinc gluconate and Manganous Chloride which were used solely and together to contradict the toxicity of silica on alveolar macrophage. Methods:Alveolar macrophages were cultured with silica ,at the same time Zinc gluconate , Manganous Chloride and Zinc gluconate together with Manganous Chloride were added respectively. Superoxide dismutase(SOD) and malodialdehyde( MDA) in alveolar macrophage,and lactic dehydrogrnase (LDH) were measured. Results:Zinc gluconate , Manganous Chloride and Zinc gluconate together with Manganous Chloride have the effects to contradict the toxicity of silica on alveolar macrophage.The most suitable combination of the two substance should be middle dosage of Zinc gluconate(1.5mg/L) with first dosage of Manganous Chloride (0.25 mg/L). Conclusion:Zinc gluconate and Manganous Chloride which were used solely and together can change MDA production and SOD activation induce by silica on alveolar macrophage.
