灿烂绿探针共振瑞利散射法测定药物及生物样品中的卡托普利

Resonance Rayleigh Scattering Method for Determination of Captopril in Drug and Biological Samples with Brilliant Green as Probe

目的建立快速测定药物及生物样品中卡托普利的共振瑞利散射(RRS)新方法。方法在p H 9.05的Tris-盐酸缓冲溶液中,以灿烂绿作探针,采用共振瑞利散射技术研究药物及生物样品中卡托普利的定量检测方法,检测波长为368 nm。结果在弱碱性Tris-盐酸介质中,卡托普利与灿烂绿反应生成绿色的二元离子缔合物,使体系的RRS发生猝灭效应,最大RRS峰位于368 nm波长处,卡托普利的质量浓度在0.005~0.43 mg·L^(-1)内与体系的RRS猝灭程度呈线性关系,检出限为0.0048mg·L^(-1)。加标回收率为98.95%~102.0%,相对标准偏差(RSD)(n=6)为1.6%~2.3%。结论方法简便、快速、灵敏,用于市售卡托普利药物及人体血液、尿液中卡托普利的测定,结果满意。

OBJECTIVE To establish a new resonance Rayleigh scattering method for the fast determination of captopril in drug and biological samples. METHODS In a Tris-hydrochloric acid buffer solution of pH 9. 05, quantitative methods of determination of captopril in drug and biological samples were studied by resonance Rayleigh scattering technology with brilliant green as probe. The de- tection wavelength was set at 368 nm. RESULTS In the weak alkaline Tris-hydrochloric acid medium, captopril reacted with brilliant green to form a green binary ionic association complex, which led to a quench effect of the system＇s resonance rayleigh scattering （RRS）. The maximum resonance Rayleigh scattering peak was located at 368 nm. At this wavelength, the system＇s RRS quenching degree was directly proportional to the mass concentration of captopril in the range of 0. 005 to 0.43 mg· L - 1 with the detection limit of 0. 004 8 mg · L-1. The recovery and RSD （n = 6） were found to be 98.95% - 102. 0% and 1.6% -2. 3%, respectively. CON- CLUSION The method is simple, rapid and sensitive, which was applied to determine the contents of captopril in commercially a- vailable captoril drugs and in human blood and urine with satisfactory results.