当归挥发油对自发性高血压大鼠ET-1、PGI2、VEGF表达水平的影响

Effects of Angelica Sinensis Oil on ET-1,PGI2 and VEGF Expression in Spontaneously Hypertensive Rats

目的:通过观察当归挥发油对自发性高血压大鼠血管内皮活性物质表达的影响来探讨其降压的作用机制。方法:将自发性高血压大鼠随机分为模型组、缬沙坦组(8 mg/kg)、当归挥发油不同剂量(3、6、12 mg/kg)组,同周龄Wistar大鼠设为正常组作对照,每组10只。模型组和正常组给予等量生理盐水,连续灌胃4周。采用无创血压检测系统测定给药前后大鼠尾动脉收缩压;给药4周后麻醉大鼠,分离胸主动脉,苏木精-伊红染色观察其内皮形态改变;腹主动脉采血,ELISA检测大鼠血清中内皮素(ET)-1、前列环素I2(PGI2)、内皮生长因子(VEGF)水平。结果:与模型组比较,当归挥发油不同剂量组收缩压呈不同程度下降,差异有统计学意义(P<0.05);苏木精-伊红染色显示:与模型组比较,各给药组均能不同程度改善胸主动脉内皮形态和内膜弹力纤维曲度,降低弹力纤维的增生;ELISA结果显示:与正常组比较,模型组ET-1、VEGF水平明显升高(P<0.05),PGI2水平明显降低(P<0.05);与模型组比较,各给药组ET-1、VEGF水平明显降低(P<0.05),PGI2水平无明显变化(P>0.05)。结论:当归挥发油具有明显的降压效果,对胸主动脉内皮具有保护作用,其降压作用可能与抑制血清中的ET-1、VEGF水平有关。

Objective： To observe the effects of volatile oil of angelica on the vascular endothelial activity of substances in spontaneously hypertensive rats and to explore its antihypertensive mechanism of action. Method：The spontaneous hypertensive rats were randomly divided into model group, valsartan group（8 mg/kg）, angelica sinensis volatile oil different dose groups （3 mg/kg, 6 mg/kg, and 12 mg/kg） ,and Wistar rats of the same age as the normal control group,each group of 10. The model group and the normal group were given the same amount of saline,gavage for 4 weeks. The noninvasive blood pressure detection system was used to measure the systolic pressure of the caudal artery in rats before and after administration. Four weeks after the administration, the rats were anesthetized and the thoracic aorta was isolated and the morphology of the endothelium was observed by HE staining. Serum levels of endothelin 1 （ET-1）, prostacyclin I2（PGI2）, and endothelial growth factor （VEGF） were measured by ELISA. Result：Compared with the model group, systolic blood pressure decreased to varying degrees in the angelica volatile oil different dose groups, the difference was statistically significant（P〈0.05. And the mor- phology of endothelium of thoracic aorta and the curvature of endometrial elastic fiber were improved and the proliferation of elastic fibers were reduced after treatment with different dose of angelica volatile oil. Meanwhile, the increased levels of ET-1 and VEGF were significantly recovered（P〈0.05）but reduced PGI2 level remained no change after treatment with angelica volatile oil（P〉0.05）. Conclusion： Angelica essential oil has a significant anti-hypertensive effect,thoracic aorta endothelial protection, the antihypertensive effect may be related to the inhibition of serum ET-1 and VEGF levels.