非小细胞肺癌患者血清中miR-25的表达水平及筛查价值

Expression levels of serum miR-25 in the patients with non-small cell lung cancer and its clinical significance

目的检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清miR-25的表达水平,探讨其对NSCLC的筛查价值。方法采集南京军区南京总医院82例未经治疗的NSCLC患者(TNMⅠ期4例、Ⅱ期10例、Ⅲ期11例、Ⅳ期53例及未知者4例)和82例年龄、性别匹配的健康人对照血清样本,实时荧光定量PCR法(qRT-PCR)检测患者及对照血清miR-25的表达水平,并通过ROC曲线评估血清miR-25对NSCLC的筛查价值。结果 NSCLC患者血清miR-25的表达水平明显高于健康人对照组(0.017±0.028 vs 0.004±0.004,t=4.098,P<0.01)。血清miR-25用于筛查NSCLC的ROC曲线下面积(AUCROC)为0.818(95%CI:0.753~0.882),敏感性为70.7%,特异性为80.7%;血清miR-25筛查Ⅰ/Ⅱ期NSCLC患者的AUCROC为0.852(95%CI:0.728~0.976),敏感性和特异性分别为78.6%和87.8%。Logistic回归分析显示,miR-25是NSCLC的独立危险因素(OR=10.84,95%CI:5.07~23.19,P<0.01)。结论 NSCLC患者血清中miR-25表达水平升高,有望成为NSCLC辅助筛查的新型分子标志物。

Objective To determine the expression levels of serum miR-25 in the patients with non-small cell lung cancer（ NSCLC）,and evaluate its clinical significance in the screening of NSCLC. Methods Serum samples from 82 untreated NSCLC patients,including 4 with TNM satge Ⅰ,10 with TNM stage Ⅱ,11 with TNM stage Ⅲ,53 with TNM stage Ⅳ and 4 with unknown stage,and 82 healthy controls with matched age and gender were collected,and the levels of miR-25 in these samples were determined by quantitative reverse transcription PCR（ qRT-PCR）. The diagnostic value of miR-25 in NSCLC was evaluated by the receiver operating characteristic（ ROC） curve. Results Serum miR-25 levels in NSCLC patients were significantly higher than that in healthy controls（ 0. 017 ±0. 028 vs 0. 004 ± 0. 004,t = 4. 098,P〈0. 01）. The area under the ROC curve（ AUCROC）,sensitivity and specificity of miR-25 for the diagnosis of NSCLC were 0. 818（ 95% CI： 0. 753-0. 882）,70. 7% and 80. 7%,respectively. In addition,the AUCROC,sensitivity and specificity of serum miR-25 for the screening of stage Ⅰ/Ⅱ NSCLC were 0. 852（ 95% CI： 0. 728-0. 976）,78. 6% and87. 8%,respectively. Logistic regression analysis showed that miR-25 was an independent risk factor of NSCLC（ OR = 10. 84,95%CI： 5. 07-23. 19,P〈0. 01）. Conclusion Serum miR-25 levels in NSCLC patients increase significantly,indicating that it may be a novel molecular biomarker for the screening of NSCLC.