妊娠期高血压患者血清和胎盘组织miR-206的表达及意义

Expression of miR-206 in serum and placental tissue of patients with gestational hypertension and its clinical significance

目的探讨妊娠期高血压患者血清和胎盘组织中miR-206的表达水平及其临床意义。方法以34例妊娠期高血压孕产妇、26例轻度子痫前期孕产妇及27例重度子痫前期孕产妇为研究对象,另选取32例正常妊娠孕产妇作为对照组,实时荧光定量PCR(qRT-PCR)检测孕产妇血清及胎盘组织中miR-206的表达水平;收集各组的临床病理资料,并进行相关性分析。结果与对照组相比,妊娠期高血压组、轻度子痫前期组、重度子痫前期组血清及胎盘组织中miR-206的表达水平均降低(F=79.10,129.18,P均<0.05),进一步进行组间两两比较发现各组间差异均有统计学意义(P均<0.05);相关性分析结果显示,对照组、妊娠期高血压组、轻度子痫前期组及重度子痫前期组血清miR-206的表达水平与胎盘组织均呈正相关(r分别为0.402、0.512、0.416、0.397,P均<0.05);此外,血清及胎盘组织中miR-206的表达与妊娠期高血压组孕产妇收缩压、舒张压、尿蛋白水平呈明显负相关(P均<0.05),与孕产妇孕龄、清蛋白清蛋白、新生儿体重呈明显正相关(P均<0.05)。结论miR-206在妊娠期高血压患者血清及胎盘组织中明显低表达,可能参与妊娠期高血压的发生及发展。

Objective To investigate the expression levels of miR-206 in serum and placenta tissues of patients with gestational hypertension and its clinical significance. Methods The expression levels of miR-206 in serum and placental tissue samples from 34 pregnant women with gestational hypertension,26 with mild preeclampsia,27 with severe preeclampsia and 32 normal pregnant women were detected by quantitative real-time PCR（ qRT-PCR）,and their clinical pathological data were collected for correlation analysis. Results Compared with normal pregnant women,the expression levels of miR-206 in sera and placental tissues of pregnant women with gestational hypertension,mild preeclampsia or severe preeclampsia decreased significantly in turn（ all P〈0. 05）,and there was significant difference（ F = 79. 10 and 129. 18,respectively,both of P〈0. 05）. The correlation analysis showed that the expression levels of miR-206 in sera of normal pregnant women and pregnant women with gestational hypertension,mild preeclampsia or severe preeclampsia were positively related to that in placental tissues（ r = 0. 402,0. 512,0. 416 and 0. 397,respectively,all P〈0. 05）. In addition,the expression levels of miR-206 in serum and placental tissue of pregnant women with gestational hypertension were negatively related to systolic blood pressure,diastolic blood pressure and urinary protein level（ P〈0. 05）,but positively to gestational age,albumin level and neonatal body weight（ P〈0. 05）. Conclusion The expression of miR-206 in serum and placental tissue of pregnant women with gestational hypertension decreases significantly,which may be involved in the development and progression of gestational hypertension.