头花蓼对幽门螺杆菌相关性胃炎大鼠胃组织SIRT1、p53和p21的影响

Effects of Polygonum capitatum on SIRT1,p53 and p21 in Helicobacter Pylori Associated Gastritis Rats

目的:观察头花蓼对幽门螺杆菌相关性胃炎(HAG)的治疗效果及对HAG胃组织中SIRT1/p53/p21信号通路的影响。方法:30只SD大鼠均分为正常对照组、模型组和药物组,正常对照组给予无菌脑心浸液肉汤灌胃,模型组和药物组大鼠给予H.pylori灌胃(1×1011CFU/L SS2000菌液1.5 m L/只)建立HAG模型,药物组HAG模型大鼠给予1.58 mg/(kg·d)头花蓼灌胃治疗,正常对照组大鼠和模型组大鼠给予等量无菌生理盐水灌胃,连续给药14 d;治疗结束4周时检测大鼠胃组织H.pylori定植量、观察各组大鼠胃黏膜组织学改变,采用Real time PCR和Western blot技术检测大鼠胃组织SIRT1、p53及p21 mRNA及蛋白表达水平。结果:正常对照组大鼠胃组织H.pylori定植量为0,模型组和药物组大鼠胃黏膜均有H.pylori定植,与模型组比较,药物组H.pylori定植量显著降低(P<0.05);正常对照组大鼠胃组织无病理形态学改变,模型组大鼠H.pylori感染后的胃黏膜腺体排列不规则,胃黏膜固有层中有较多淋巴细胞浸润,细胞浸润多呈弥漫性;药物组大鼠胃组织病理形态学改变少于模型组;与正常对照组比较,模型组病理评分显著升高,而药物组病理评分显著低于模型组,差异有统计学意义(P<0.05);H.pylori感染后,与正常对照组相比,模型组大鼠胃组织SIRT1、p53基p21 mRNA表达量增加(P<0.05);头花蓼治疗后,与模型组相比,药物组大鼠胃组织SIRT1、p53及p21 mRNA水平都明显升高(P<0.05);与正常对照组相比,模型组大鼠胃组织SIRT1、p53及p21蛋白表达均有所升高,但差异无统计学意义(P>0.05);而头花蓼治疗后,与模型组相比,SIRT1、p53和p21蛋白表达明显升高(P<0.05)。结论:头花蓼治疗可能是通过上调SIRT1、p53和p21基因表达来发挥作用。

Objective： To observe the antibacteria function of Polygonum capitatum in Helicobacter pylori-associated gastritis（ HAG）,and to investigate whether SIRT1/p53/p21 signal pathway was influenced. Methods： Thirty rats were randomly divided into normal control group（ NC）,model control group（ MC） and drug treatment group（ DT）. Rats in group NC were given gavage of sterile brain heart infusion broth,and those of groups MC and DT were given with H. pylori（ 1 × 1011 cfu/L,1. 5 m L each rat） to establish HAG model. Rats in group DT were then given P. capitatum 1. 58 mg/（ kg·d） for treatment,while rats in groups NC and MC were given sterile normal saline for two weeks. In 4 weeks after the treatment finished,the levels of H. pylori colonization in gastric mucosa were detected and the pathological changes in gastric mucosa were observed with HE stained sections.The expression of SIRT1,p53,p21 mRNA and proteins were determined by real-time PCR and western blotting. Results： The colonization quantity of H. pylori in gastric mucosa of group NC was 0. The H. pylori level in group DT was significantly lower than that in group MC（ P 〈0. 05）. No pathological change was found in rats of group NC. In rats of group MC,after H. pylori infection,the gastric mucosa glands arranged irregularly,and there were relatively more lymphocytes diffusively infiltrated in gastric mucosa lamina propria. In group DT,the pathological scores were significantly lower than those in group MC（ P〈 0. 05）.,The expression levels of rat gastric tissue SIRT1,p53 and p21 mRNA were higher in group MC than those in group NC（ P 〈0. 05） after H. pylori infection,and were obviously higher in group DT than those in group MC（ P 〈0. 05） after treatment with P. capitatum. The expression of SIRT1,p53 and p21 protein in group MC was higher than that in group NC,but the difference was not significant（ P 〉0. 05）,and it＇s obviously higher in group DT than in group MC. Conclusions：P. capitatum has some curing effects to HAG,which might perform through the signal pathway of SIRT1/p53/p21 by up regulating expression of SIRT1/p53/p21 genes.