一种经皮种植体抗菌肽控释涂层的构建及性能研究

Construction and characterization of an antibacterial peptide controlled release coating for percutaneous implant

目的:为了提高经皮种植体的抗菌性能,在二氧化钛(TiO_2)纳米管表面构建了PDLLA复合抗菌肽的载药涂层,并对其体外缓释性能和抗菌性能进行研究。方法:阳极氧化法制备TiO_2纳米管试件,使用聚-DL-乳酸(PDLLA)作为抗菌肽HHC-36的缓释剂,采用溶剂浇铸法在二氧化钛纳米管表面制备载药涂层,电镜观察形貌,测定并绘制其缓释曲线;采用抑菌圈法检测载药试件的抗菌性能。结果:电镜下显示TiO_2纳米管的管径为80~120 nm,加载药物后的试件表面可见药物覆盖,载药试件体外缓释测定有15 d以上持续释放,抗菌性能检测有持续10 d以上15 mm左右抑菌圈。结论:在TiO_2纳米管表面成功制备了PDLLA复合HHC-36抗菌肽的缓释涂层,该载药涂层表现出良好的缓释性能和抗菌性能。

Objective： To construct an antibacterial peptide controlled release coating for percutaneous implant and to study its release and antibacterial properties. Methods： TiO2 nanotube specimens were prepared by anodizing. PDLLA was used as the retarder and HHC-36 as the antibacterial peptide, PDLLA-HHC-36 were loaded on TiO2 nanotube surface by solvent-casting technology. The sur- face form of the specimens was observed under SEM. Sustained release was analyzed by the release curves and the antibacterial effect was examined with inhibition zone test. Results： TiO2 nanotube specimens with 80 - 120 nm diameter were fabricated, the drug coating was observed under SEM. The drug loaded specimens showed 15 days sustained release in vitro and inhibition zone about 15 mm in di- ameter was found in the test for at least 10 days. Conclusion： TiO2 nanotube specimens with PDLLA-HHC-36 controlled release coat- ing has sustained-release and antibacterial properties.